Human inhibitor of growth family, member 4 ELISA Kit | ING4 elisa kit
Human Inhibitor of growth protein 4, ING4 ELISA Kit
Gene Names
ING4; my036; p29ING4
Reactivity
Human
Synonyms
inhibitor of growth family, member 4; N/A; Human Inhibitor of growth protein 4, ING4 ELISA Kit; Human Inhibitor of growth protein 4 (ING4) ELISA kit; MGC12557; my036; p29ING4; brain my036 protein; candidate tumor suppressor p33 ING1 homolog; inhibitor of growth family; member 4; ING4 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human ING4. No significant cross-reactivity or interference between human ING4 and analogues was observed.
Sequence Length
249
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
47 pg/ml -3000 pg/ml.
Sensitivity
The minimum detectable dose of human ING4 is typically less than 11.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for ING4 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ING4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ING4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ING4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ING4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,530 Da
NCBI Official Full Name
inhibitor of growth protein 4 isoform 9
NCBI Official Synonym Full Names
inhibitor of growth family, member 4
NCBI Official Symbol
ING4
NCBI Official Synonym Symbols
my036; p29ING4
NCBI Protein Information
inhibitor of growth protein 4; brain my036 protein; candidate tumor suppressor p33 ING1 homolog
UniProt Protein Name
Inhibitor of growth protein 4
UniProt Gene Name
ING4
UniProt Synonym Gene Names
My036
UniProt Entry Name
ING4_HUMAN