Rat amyloid beta (A4) precursor protein ELISA Kit | APP elisa kit
Rat Amyloid beta A4 protein, APP ELISA Kit
Reactivity
Rat
Synonyms
amyloid beta (A4) precursor protein; N/A; Rat Amyloid beta A4 protein, APP ELISA Kit; Rat Amyloid beta A4 protein (APP) ELISA kit; AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN2; OTTHUMP00000096096; amyloid beta A4 protein; beta-amyloid peptide; cerebral vascular amyloid peptide; peptidase nexin-II; protease nexin-II; APP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat APP. No significant cross-reactivity or interference between rat APP and analogues was observed.
Sequence Length
58
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich
Detection Range
15.6 ng/ml-1000 ng/ml.
Sensitivity
Typically less than 3.9 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for APP elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APP bound in the initial step. The color development is stopped and the intensity of the color is measured.