Monkey amyloid beta A4 protein ELISA Kit | APP elisa kit

Monkey amyloid beta A4 protein ELISA Kit

Cat. Num. AAA27296

• Gene Names: APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• Reactivity: Monkey
• Synonyms: amyloid beta A4 protein; Monkey amyloid beta A4 protein ELISA Kit; APP elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of AbetaA4. No significant cross-reactivity or interference between AbetaA4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AbetaA4 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for APP elisa kit

Principle of the Assay: AbetaA4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AbetaA4 antibody and an AbetaA4-HRP conjugate. The assay sample and buffer are incubated together with AbetaA4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AbetaA4 concentration since AbetaA4 from samples and AbetaA4-HRP conjugate compete for the anti-AbetaA4 antibody binding site. Since the number of sites is limited, as more sites are occupied by AbetaA4 from the sample, fewer sites are left to bind AbetaA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AbetaA4 concentration in each sample is interpolated from this standard curve.

Intended Uses: This AbetaA4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey AbetaA4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for APP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4502167
• NCBI GeneID: 351
• NCBI Accession #: NP_000475.1
• NCBI GenBank Nucleotide #: NM_000484.3
• Molecular Weight: 84,521 Da
• NCBI Official Full Name: amyloid beta A4 protein isoform a
• NCBI Official Synonym Full Names: amyloid beta (A4) precursor protein
• NCBI Official Symbol: APP
• NCBI Official Synonym Symbols: AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• NCBI Protein Information: amyloid beta A4 protein; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); alzheimer disease amyloid protein; cerebral vascular amyloid peptide
• UniProt Protein Name: Amyloid beta A4 protein
• UniProt Gene Name: APP
• UniProt Synonym Gene Names: A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50)
• UniProt Entry Name: A4_HUMAN