SDS-PAGE
Tetracycline repressor protein class B from transposon Tn10 (tetR) Recombinant Protein | tetR recombinant protein
Recombinant Escherichia coli Tetracycline repressor protein class B from transposon Tn10 (tetR)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Tetracycline repressor protein class B from transposon Tn10 (tetR); Recombinant Escherichia coli Tetracycline repressor protein class B from transposon Tn10 (tetR); tetR recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-207aa; Full-Length
Sequence
MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for tetR recombinant protein
TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
References
Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant.Postle K., Nguyen T.T., Bertrand K.P.Nucleic Acids Res. 12:4849-4863(1984)
A threonine to alanine exchange at position 40 of Tet repressor alters the recognition of the sixth base pair of tet operator from GC to AT.Altschmied L., Baumeister R., Pfleiderer K., Hillen W.EMBO J. 7:4011-4017(1988)
Overlapping divergent promoters control expression of Tn10 tetracycline resistance.Bertrand K.P., Postle K., Wray L.V. Jr., Reznikoff W.S.Gene 23:149-156(1983)
Mutations in the Tn10 tet repressor that interfere with induction. Location of the tetracycline-binding domain.Smith L.D., Bertrand K.P.J. Mol. Biol. 203:949-959(1988)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39.4 kDa
NCBI Official Full Name
MULTISPECIES: transposase
NCBI Official Symbol
D616_p109083
NCBI Protein Information
tetracycline repressor protein
UniProt Protein Name
Tetracycline repressor protein class B from transposon Tn10
UniProt Gene Name
tetR
UniProt Entry Name
TETR2_ECOLX
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Product Notes
The tetR tetr (Catalog #AAA18582) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-207aa; Full-Length. The amino acid sequence is listed below: MSRLDKSKVI NSALELLNEV GIEGLTTRKL AQKLGVEQPT LYWHVKNKRA LLDALAIEML DRHHTHFCPL EGESWQDFLR NNAKSFRCAL LSHRDGAKVH LGTRPTEKQY ETLENQLAFL CQQGFSLENA LYALSAVGHF TLGCVLEDQE HQVAKEERET PTTDSMPPLL RQAIELFDHQ GAEPAFLFGL ELIICGLEKQ LKCESGS. It is sometimes possible for the material contained within the vial of "Tetracycline repressor protein class B from transposon Tn10 (tetR), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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