Mouse cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit | CYFRA21-1 elisa kit

Mouse cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit

Cat. Num. AAA18965

• Reactivity: Mouse
• Synonyms: cytokeratin fragment antigen 21-1 (CYFRA21-1); N/A; Mouse cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit; CYFRA21-1 elisa kit

• Reactivity: Mouse
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
• Detection Range: 0.05-10ng/ml
• Sensitivity: 0.01ng/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for CYFRA21-1 elisa kit

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CYFRA21-1 antibody. CYFRA21-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CYFRA21-1 Antibody is added and binds to CYFRA21-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYFRA21-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CYFRA21-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse cytokeratin fragment antigen 21-1 (also known as CYFRA21-1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.