Human cytokeratin fragment antigen 21-1, CYFRA21-1 ELISA Kit | CYFRA21-1 elisa kit
Human cytokeratin fragment antigen 21-1, CYFRA21-1 ELISA Kit
Reactivity
Human
Synonyms
cytokeratin fragment antigen 21-1, CYFRA21-1; N/A; Human cytokeratin fragment antigen 21-1, CYFRA21-1 ELISA Kit; CYFRA21-1 elisa kit
Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
Detection Range
0.1-40ng/ml
Sensitivity
0.051ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for CYFRA21-1 elisa kit
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CYFRA21-1 antibody. CYFRA21-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYFRA21-1 Antibody is added and binds to CYFRA21-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYFRA21-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CYFRA21-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human cytokeratin fragment antigen 21-1 (also known as CYFRA21-1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human cytokeratin fragment antigen 21-1 (also known as CYFRA21-1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.