Rabbit SSRP1 Polyclonal Antibody | anti-SSRP1 antibody

Anti-SSRP1 Antibody Picoband

Cat. Num. AAA19778

• Gene Names: SSRP1; FACT; T160; FACT80
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: SSRP1; Polyclonal Antibody; Anti-SSRP1 Antibody Picoband; Homeobox protein SIX3; Sine oculis homeobox homolog 3; SIX3; anti-SSRP1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Highly expressed in placenta, lung, kidney, testis and ovary. WeaklyExpressed in spleen and thymus. NotExpressed in peripheral blood lymphocytes. Detected in hippocampus.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Applicable Applications for anti-SSRP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml, Human, Mouse, Rat; IHC(Paraffin-embedded Section): 2-5ug/ml, Human; ICC/IF: 5ug/ml, Human; FC/FACS (Fixed): 1-3ug/1x10⁶ cells, Human; ELISA: 0.1-0.5ug/ml
• Immunogen: E coli-derived human SSRP1 recombinant protein (Position: A2-R381).
• Subcellular Localization: Nucleus
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
• Cross Reactivity: No cross-reactivity with other proteins.
• Protein Function: Transcriptional regulator which can act as both a transcriptional repressor and activator by binding a ATTA homeodomain core recognition sequence on these target genes. During forebrain development represses WNT1 expression allowing zona limitans intrathalamica formation and thereby ensuring proper anterio-posterior patterning of the diencephalon and formation of the rostral diencephalon. Acts as a upstream activator of SHH expression in the rostral diencephalon ventral midline and that in turn SHH maintains its expression. In addition, Six3 activity is required for the formation of the telencephalon. During postnatal stages of brain development is necessary for ependymal cell maturation by promoting the maturation of radial glia into ependymal cells through regulation of neuroblast proliferation and migration. Acts on the proliferation and differentiation of neural progenitor cells through activating transcription of CCND1 AND CCND2. During early lens formation plays a role in lens induction and specification by activating ly PAX6 in the presumptive lens ectoderm. In turn PAX6 activates SIX3 resulting in activation of PDGFRA and CCND1 promoting cell proliferation. Also is required for the neuroretina development by ly suppressing WNT8B expression in the anterior neural plate territory. Its action during retina development and lens morphogenesis is AES and TLE4-dependent manner. Furthermore, during eye development regulates several genes expression. Before and during early lens development represses the CRYGF promoter by binding a SIX repressor element. ly activates RHO transcription, or cooperates with CRX or NRL. Six3 functions also in the formation of the proximodistal axis of the optic cup, and promotes the formation of optic vesicles-like structures. During pituitary development, acts in parallel or alternatively with HESX1 to control cell proliferation through Wnt/beta-catenin pathway (By similarity). Plays a role in eye development by suppressing WNT1 expression and in dorsal- ventral patterning by repressing BMP signaling pathway.
• Preparation and Storage: At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 8. Flow Cytometry analysis of U251 cells using anti-SSRP1 antibody (AAA19778).Overlay histogram showing U251 cells stained with AAA19778 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSRP1 Antibody (AAA19778, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence)

(Figure 7. IF analysis of SSRP1 using anti-SSRP1 antibody (AAA19778) and anti-Tubulin Alpha antibody (M03989-3).SSRP1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSRP1 Antibody (AAA19778) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSRP1 Antibody (AAA19778) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSRP1 Antibody (AAA19778) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSRP1 Antibody (AAA19778) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSRP1 Antibody (AAA19778) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human DLD-1 whole cell lysates,Lane 3: human MOLT-4 whole cell lysates,Lane 4: monkey COS-7 whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse testis tissue lysate,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSRP1 antigen affinity purified polyclonal antibody (#AAA19778) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SSRP1 at approximately 92 kDa. The expected band size for SSRP1 is at 81 kDa.)

Related Product Information for anti-SSRP1 antibody

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Categories/Family for anti-SSRP1 antibody

Primary Antibodies; Developmental Families; Domain Families; Epigenetics and Nuclear Signaling; Neurogenesis; Neurology Process; Neuroscience; Transcription

References

1. Belotserkovskaya, R., Oh, S., Bondarenko, V. A., Orphanides, G., Studitsky, V. M., Reinberg, D. FACT facilitates transcription-dependent nucleosome alteration. Science 301: 1090-1093, 2003. 2. Bruhn, S. L., Pil, P. M., Essigmann, J. M., Housman, D. E., Lippard, S. J. Isolation and characterization of human cDNA clones encoding a high mobility group box protein that recognizes structural distortions to DNA caused by binding of the anticancer agent cisplatin. Proc. Nat. Acad. Sci. 89: 2307-2311, 1992. 3. Keller, D. M., Zeng, X., Wang, Y., Zhang, Q. H., Kapoor, M., Shu, H., Goodman, R., Lozano, G., Zhao, Y., Lu, H. A DNA damage-induced p53 serine 392 kinase complex contains CK2, hSpt16, and SSRP1. Molec. Cell 7: 283-292, 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 730840
• NCBI GeneID: 6749
• UniProt Accession #: Q08945; Q5BJG8
• Molecular Weight: 709
• NCBI Official Full Name: FACT complex subunit SSRP1
• NCBI Official Synonym Full Names: structure specific recognition protein 1
• NCBI Official Symbol: SSRP1
• NCBI Official Synonym Symbols: FACT; T160; FACT80
• NCBI Protein Information: FACT complex subunit SSRP1; hSSRP1; FACTp80; cisplatin-DNA SSRP; FACT 80 kDa subunit; high mobility group box; structure-specific recognition protein 1; facilitates chromatin remodeling 80 kDa subunit; recombination signal sequence recognition protein 1
• UniProt Protein Name: FACT complex subunit SSRP1
• UniProt Gene Name: SSRP1
• UniProt Synonym Gene Names: FACT80; FACT 80 kDa subunit; FACTp80; hSSRP1
• UniProt Entry Name: SSRP1_HUMAN