Mouse Eosinophil Peroxidase (EPX) ELISA Kit | EPX elisa kit
Mouse Eosinophil Peroxidase (EPX) ELISA Kit
Gene Names
EPX; EPO; EPP; EPX-PEN
Reactivity
Mouse
Synonyms
Eosinophil Peroxidase (EPX); N/A; Mouse Eosinophil Peroxidase (EPX) ELISA Kit; EPO; EPP; EPX-PEN; EPER; EPX elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of EPX. No significant cross-reactivity or interference between EPX and analogues was observed.
Sequence Length
715
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.117ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EPX were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EPX were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for EPX elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EPX in mouse serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to EPX. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to EPX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EPX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EPX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to EPX. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to EPX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EPX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EPX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for EPX elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
81,040 Da
NCBI Official Full Name
eosinophil peroxidase
NCBI Official Synonym Full Names
eosinophil peroxidase
NCBI Official Symbol
EPX
NCBI Official Synonym Symbols
EPO; EPP; EPX-PEN
NCBI Protein Information
eosinophil peroxidase
UniProt Protein Name
Eosinophil peroxidase
UniProt Gene Name
EPX
UniProt Synonym Gene Names
EPER; EPO; EPP; EPO
UniProt Entry Name
PERE_HUMAN
