Human Eosinophil peroxidase (EPX) ELISA Kit | EPX elisa kit

Human Eosinophil peroxidase (EPX) ELISA Kit

Cat. Num. AAA27196

• Gene Names: EPX; EPO; EPP; EPX-PEN
• Reactivity: Human
• Synonyms: Eosinophil peroxidase (EPX); Human Eosinophil peroxidase (EPX) ELISA Kit; EPX elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of EPX. No significant cross-reactivity or interference between EPX and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EPX and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for EPX elisa kit

Principle of the Assay: EPX ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-EPX antibody and an EPX-HRP conjugate. The assay sample and buffer are incubated together with EPX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPX concentration since EPX from samples and EPX-HRP conjugate compete for the anti-EPX antibody binding site. Since the number of sites is limited, as more sites are occupied by EPX from the sample, fewer sites are left to bind EPX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPX concentration in each sample is interpolated from this standard curve.

Intended Uses: This EPX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human EPX. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 182146
• NCBI GeneID: 8288
• Molecular Weight: 81,040 Da
• NCBI Official Full Name: eosinophil peroxidase
• NCBI Official Synonym Full Names: eosinophil peroxidase
• NCBI Official Symbol: EPX
• NCBI Official Synonym Symbols: EPO; EPP; EPX-PEN
• NCBI Protein Information: eosinophil peroxidase
• UniProt Protein Name: Eosinophil peroxidase
• UniProt Gene Name: EPX
• UniProt Synonym Gene Names: EPER; EPO; EPP; EPO
• UniProt Entry Name: PERE_HUMAN