Human Toll Like Receptor 5 (TLR5) ELISA Kit | TLR5 elisa kit
Human Toll Like Receptor 5 (TLR5) ELISA Kit
Gene Names
TLR5; SLE1; TIL3; SLEB1; MELIOS
Reactivity
Human
Synonyms
Toll Like Receptor 5 (TLR5); N/A; Human Toll Like Receptor 5 (TLR5) ELISA Kit; CD285; SLEB1; TIL3; Toll/Interleukin-1 Receptor-Like Protein 3; TLR5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TLR5. No significant cross-reactivity or interference between TLR5 and analogues was observed.
Sequence Length
858
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
0.78-50ng/mL
Sensitivity
0.30ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TLR5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TLR5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for TLR5 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TLR5 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TLR5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TLR5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TLR5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TLR5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TLR5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TLR5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TLR5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TLR5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for TLR5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
97,834 Da
NCBI Official Full Name
Toll-like receptor 5
NCBI Official Synonym Full Names
toll-like receptor 5
NCBI Official Symbol
TLR5
NCBI Official Synonym Symbols
SLE1; TIL3; SLEB1; MELIOS
NCBI Protein Information
toll-like receptor 5
UniProt Protein Name
Toll-like receptor 5
UniProt Gene Name
TLR5
UniProt Synonym Gene Names
TIL3
UniProt Entry Name
TLR5_HUMAN
