Human Toll Like Receptor 1 (TLR1) ELISA Kit | TLR1 elisa kit
Human Toll Like Receptor 1 (TLR1) ELISA Kit
Gene Names
TLR1; TIL; CD281; rsc786; TIL. LPRS5
Reactivity
Human
Synonyms
Toll Like Receptor 1 (TLR1); N/A; Human Toll Like Receptor 1 (TLR1) ELISA Kit; CD281; TIL; Rsc786; Toll/interleukin-1 receptor-like protein; TLR1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TLR1. No significant cross-reactivity or interference between TLR1 and analogues was observed.
Sequence Length
786
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.127ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TLR1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TLR1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for TLR1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TLR1 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TLR1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TLR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TLR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TLR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TLR1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TLR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TLR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TLR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for TLR1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
90,291 Da
NCBI Official Full Name
Toll-like receptor 1
NCBI Official Synonym Full Names
toll-like receptor 1
NCBI Official Symbol
TLR1
NCBI Official Synonym Symbols
TIL; CD281; rsc786; TIL. LPRS5
NCBI Protein Information
toll-like receptor 1
UniProt Protein Name
Toll-like receptor 1
UniProt Gene Name
TLR1
UniProt Synonym Gene Names
KIAA0012; TIL
UniProt Entry Name
TLR1_HUMAN
