Human Fibroblast Activation Protein Alpha (FAPa) ELISA Kit | FAPa elisa kit
Human Fibroblast Activation Protein Alpha (FAPa) ELISA Kit
Gene Names
FAP; FAPA; SIMP; DPPIV
Reactivity
Human
Synonyms
Fibroblast Activation Protein Alpha (FAPa); N/A; Human Fibroblast Activation Protein Alpha (FAPa) ELISA Kit; APCE; SIMP; DPPIV; Seprase; 170 kDa melanoma membrane-bound gelatinase; Post-proline cleaving enzyme; Serine integral membrane protease; Surface-expressed protease; FAPa elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of FAM3D. No significant cross-reactivity or interference between FAM3D and analogues was observed.
Sequence Length
239
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.78-50ng/mL
Sensitivity
0.29ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FAM3D were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FAM3D were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for FAPa elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FAM3D in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FAM3D. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FAM3D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FAM3D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FAM3D in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FAM3D. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FAM3D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FAM3D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FAM3D in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for FAPa elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26,954 Da
NCBI Official Full Name
fibroblast activation protein, alpha, isoform CRA_b
NCBI Official Synonym Full Names
fibroblast activation protein, alpha
NCBI Official Symbol
FAP
NCBI Official Synonym Symbols
FAPA; SIMP; DPPIV
NCBI Protein Information
prolyl endopeptidase FAP
UniProt Protein Name
Prolyl endopeptidase FAP
UniProt Gene Name
FAP
UniProt Synonym Gene Names
APCE
UniProt Entry Name
SEPR_HUMAN
