Rat alpha-Smooth muscle actin (alpha-SMA) ELISA Kit | alpha-SMA elisa kit
Rat alpha-Smooth muscle actin (alpha-SMA) ELISA Kit
Gene Names
ACTG2; ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
Reactivity
Rat
Synonyms
alpha-Smooth muscle actin (alpha-SMA); N/A; Rat alpha-Smooth muscle actin (alpha-SMA) ELISA Kit; Rat a-Smooth muscle actin (a-SMA) ELISA Kit; alpha-SMA elisa kit
Reactivity
Rat
Sequence Length
376
Assay Type
Sandwich
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for alpha-SMA elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat alpha-SMA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,083 Da
NCBI Official Full Name
actin, gamma-enteric smooth muscle isoform 1
NCBI Official Synonym Full Names
actin, gamma 2, smooth muscle, enteric
NCBI Official Symbol
ACTG2
NCBI Official Synonym Symbols
ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
NCBI Protein Information
actin, gamma-enteric smooth muscle; alpha-actin-3; actin-like protein
UniProt Protein Name
Actin, gamma-enteric smooth muscle
UniProt Gene Name
ACTG2
UniProt Synonym Gene Names
ACTA3; ACTL3; ACTSG
UniProt Entry Name
ACTH_HUMAN