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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)

Rabbit anti-Human Hepatoma Derived Growth Factor Polyclonal Antibody | anti-HDGF antibody

Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)

Gene Names
HDGF; HMG1L2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Hepatoma Derived Growth Factor; Polyclonal Antibody; Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF); anti-HDGF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HDGF. It has beenselected for its ability to recognize HDGF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- Y KCGDLVFAKM KGYPHWPARI DEMPEAAVKS TANKYQVFFF GTHETAFLGP KDLFPYEESK EKFGKPNKRK GFSEGLWEIE NNPTVKASGY QSSQKKSCVE EPEPEPEAAE GDGDKKGNAE GSSDEEGKLV IDEPAKEKNE KGALKRRAGD LLEDSPKRPK EAENPEGEEK EAATLEVERP LPMEVEKNST PSEPGSGRGP
Sequence Length
189
Applicable Applications for anti-HDGF antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-5ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
HDGF (Tyr10~Leu240)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant HDGF (Tyr10~Leu240) expressed in E Coli.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)

IHC (Immunohistchemistry)

(FITC staining on IHC-P Simple: A549 cells.)

IHC (Immunohistchemistry) (FITC staining on IHC-P Simple: A549 cells.)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Human Stomach Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Stomach Tissue)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Human Kidney Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Kidney Tissue.)

WB (Western Blot)

(Western Blot: Sample: Recombinant HDGF, Human.)

WB (Western Blot) (Western Blot: Sample: Recombinant HDGF, Human.)

WB (Western Blot)

(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human A549 Cells; Lane3: Human Jurkat Cells; Lane4: Human 293T Cells.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human A549 Cells; Lane3: Human Jurkat Cells; Lane4: Human 293T Cells.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,264 Da
NCBI Official Full Name
hepatoma-derived growth factor isoform b
NCBI Official Synonym Full Names
heparin binding growth factor
NCBI Official Symbol
HDGF
NCBI Official Synonym Symbols
HMG1L2
NCBI Protein Information
hepatoma-derived growth factor
UniProt Protein Name
Hepatoma-derived growth factor
UniProt Gene Name
HDGF
UniProt Synonym Gene Names
HMG1L2; HDGF; HMG-1L2

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Product Notes

The HDGF hdgf (Catalog #AAA20850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hepatoma Derived Growth Factor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-5ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the HDGF hdgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- Y KCGDLVFAKM KGYPHWPARI DEMPEAAVKS TANKYQVFFF GTHETAFLGP KDLFPYEESK EKFGKPNKRK GFSEGLWEIE NNPTVKASGY QSSQKKSCVE EPEPEPEAAE GDGDKKGNAE GSSDEEGKLV IDEPAKEKNE KGALKRRAGD LLEDSPKRPK EAENPEGEEK EAATLEVERP LPMEVEKNST PSEPGSGRGP. It is sometimes possible for the material contained within the vial of "Hepatoma Derived Growth Factor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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