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IHC (Immunohistochemistry)
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Rabbit Protein Kinase C Iota Polyclonal Antibody | anti-PKCi antibody

Protein Kinase C Iota (PKCi) Polyclonal Antibody

Gene Names
PRKCI; PKCI; DXS1179E; nPKC-iota
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Protein Kinase C Iota, Antibody; Protein Kinase C Iota (PKCi) Polyclonal Antibody; PKC-I; PRKCI; PRKC-I; NPKC-Iota; Atypical protein kinase C-lambda/iota; anti-PKCi antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PKCi. It has been selected for its ability to recognize PKCi in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
587
Applicable Applications for anti-PKCi antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Fragment
PKCi (Ile375~Val596)
Quality Control
Content: The quality control contains recombinant PKCi (Ile375~Val596) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Human Lung Cancer Tissue)

IHC (Immunohistchemistry)

(FITC staining on IHC-P Simple: Mcf7 cells))

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Human Kidney Tissue.)

WB (Western Blot)

(Western Blot: Sample: Recombinant PKCi, Human.)

WB (Western Blot)

(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human MCF7 Cells.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68,262 Da
NCBI Official Full Name
protein kinase C iota type
NCBI Official Synonym Full Names
protein kinase C iota
NCBI Official Symbol
PRKCI
NCBI Official Synonym Symbols
PKCI; DXS1179E; nPKC-iota
NCBI Protein Information
protein kinase C iota type
UniProt Protein Name
Protein kinase C iota type
UniProt Gene Name
PRKCI
UniProt Synonym Gene Names
DXS1179E; PRKC-lambda/iota; aPKC-lambda/iota

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Product Notes

The PKCi prkci (Catalog #AAA21029) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Protein Kinase C Iota (PKCi) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Kinase C Iota can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PKCi prkci for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Kinase C Iota, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.