Human Atrial Natriuretic Peptide (ANP) ELISA Kit | ANP elisa kit

Human Atrial Natriuretic Peptide (ANP) ELISA Kit

Cat. Num. AAA21065

• Gene Names: NPPA; ANF; ANP; CDD; CDP; PND; ATFB6; ATRST2; CDD-ANF
• Reactivity: Human
• Synonyms: Atrial Natriuretic Peptide (ANP); Human Atrial Natriuretic Peptide (ANP) ELISA Kit; ANH; ANF; PND; NPPA; CDD-ANF; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Natriuretic Peptide Precursor A; Prepronatriodilatin; Cardiodilatin-related peptide; ANP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ANP. No significant cross-reactivity or interference between ANP and analogues was observed.
• Sequence Length: 151
• Assay Type: Quantitative Competitive
• Samples: Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 12.35-1,000pg/mL
• Sensitivity: < 4.84pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ANP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ANP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for ANP elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific ANP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ANP and unlabeled ANP (Standards or samples) with the pre-coated antibody specific to ANP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ANP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ANP in the sample.

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ANP in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Product Categories/Family for ANP elisa kit

Endocrinology; Cardiovascular biology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 178638
• NCBI GeneID: 4878
• Molecular Weight: 16,708 Da
• NCBI Official Full Name: atrial natriuretic peptide
• NCBI Official Synonym Full Names: natriuretic peptide A
• NCBI Official Symbol: NPPA
• NCBI Official Synonym Symbols: ANF; ANP; CDD; CDP; PND; ATFB6; ATRST2; CDD-ANF
• NCBI Protein Information: natriuretic peptides A
• UniProt Protein Name: Natriuretic peptides A
• UniProt Gene Name: NPPA
• UniProt Synonym Gene Names: ANP; PND; CDD; CDP; ANF; ANP