Rat NF-kappaB p65 ELISA Kit | NF-kappaB p65 elisa kit

Rat NF-kappaB p65(Nuclear Factor-kappaB P65) ELISA Kit

Cat. Num. AAA21580

• Gene Names: NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• Reactivity: Rat
• Synonyms: NF-kappaB p65; N/A; Rat NF-kappaB p65(Nuclear Factor-kappaB P65) ELISA Kit; NF-kappaB p65 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes Rat NFKB-p65 in samples. No significant cross-reactivity or interference between Rat NFKB-p65 and analogues was observed
• Sequence Length: 969
• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.13-5000 pg/mL
• Sensitivity: 46.88 pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NFKB-p65 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NFKB-p65 were tested on 3 different plates, 20 replicates in each plate, respectively.
• Preparation and Storage: Store at 4 degree C.

Standard Curve (Sample)

Related Product Information for NF-kappaB p65 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat NFKB-p65 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NFKB-p65. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NFKB-p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat NFKB-p65. You can calculate the concentration of Rat NFKB-p65 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 34577122
• NCBI GeneID: 4790
• NCBI Accession #: NP_003989.2
• NCBI GenBank Nucleotide #: NM_003998.3
• UniProt Accession #: P19838; Q68D84; Q86V43; Q8N4X7; Q9NZC0; A8K5Y5; B3KVE8
• Molecular Weight: 85,520 Da
• NCBI Official Full Name: nuclear factor NF-kappa-B p105 subunit isoform 1
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• NCBI Official Symbol: NFKB1
• NCBI Official Synonym Symbols: p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• NCBI Protein Information: nuclear factor NF-kappa-B p105 subunit; DNA binding factor KBF1; DNA-binding factor KBF1; NF-kappabeta; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA binding subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p105 subunit
• UniProt Gene Name: NFKB1
• UniProt Entry Name: NFKB1_HUMAN