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IF (Immunofluorescence)
(Immunofluorescence analysis of Rat testis cells using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
Rabbit GPX4 Polyclonal Antibody | anti-GPX4 antibody
(KD Validated) GPX4 Polyclonal Antibody
Gene Names
GPX4; MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity purification
Synonyms
GPX4; Polyclonal Antibody; (KD Validated) GPX4 Polyclonal Antibody; GPx-4; GSHPx-4; MCSP; PHGPx; SMDS; snGPx; snPHGPx; glutathione peroxidase 4; anti-GPX4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-GPX4 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant fusion protein of human GPX4
Preparation and Storage
Storage: -20 degree C/One year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of Rat testis cells using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of Rat testis cells using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of Mouse testis cells using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of Mouse testis cells using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of rat testis cells using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of rat testis cells using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse testis cells using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse testis cells using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and GPX4 knockdown (KD) U-87MG cells using GPX4 Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and GPX4 knockdown (KD) U-87MG cells using GPX4 Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of Jurkat cells using GPX4 Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of Jurkat cells using GPX4 Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines using GPX4 Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines using GPX4 Polyclonal Antibody at 1:1000 dilution.)
Related Product Information for anti-GPX4 antibody
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.
Product Categories/Family for anti-GPX4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 22kDa
Calculated MW: 19kDa/22kDa
Calculated MW: 19kDa/22kDa
NCBI Official Full Name
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
NCBI Official Synonym Full Names
glutathione peroxidase 4
NCBI Official Symbol
GPX4
NCBI Official Synonym Symbols
MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
NCBI Protein Information
phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase
UniProt Protein Name
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
UniProt Gene Name
GPX4
UniProt Synonym Gene Names
PHGPx; GPx-4; GSHPx-4
UniProt Entry Name
GPX4_HUMAN
Similar Products
Product Notes
The GPX4 gpx4 (Catalog #AAA22318) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (KD Validated) GPX4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPX4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the GPX4 gpx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPX4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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