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IF (Immunofluorescence)
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Mouse beta actin Monoclonal Antibody | anti-ACTB antibody

beta actin Monoclonal Antibody

Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Protein A/G Purification
Synonyms
beta actin, Antibody; beta actin Monoclonal Antibody; ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; Lmnb1; LMNB1; MGC111419; OTTHUMP00000159218; anti-ACTB antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3A9
Purity/Purification
Protein A/G Purification
Form/Format
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-ACTB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 12000-1:4000
IHC: 1:200-1:400
IF: 1:200-1:400
Immunogen
Recombinant human Beta actin protein by E coli
Preparation and Storage
Store at -20 degree C Valid for 12 months. Avoid freeze/thaw cycles. Protected from prolonged exposure to light.

IF (Immunofluorescence)

(Immunofluorescence analysis of C6 cells using beta actin Monoclonal Antibody at dilution of 1:200.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using beta actin Monoclonal Antibody at dilution of 1:200.)

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cells using beta actin Monoclonal Antibody at dilution of 1:200.)

IF (Immunofluorescence)

(Immunofluorescence analysis of U-2 OS cells using beta actin Monoclonal Antibody at dilution of 1:200.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Human Lung cancer using beta actin Monoclonal Antibody at dilution of 1:1000.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Human Stomach cancer using beta actin Monoclonal Antibody at dilution of 1:1000.)

WB (Western Blot)

(Western blot with Anti beta actin Monoclonal Antibody at dilution of 1:3000. Lane 1: Raji cell lysate, Lane 2: Jurkat cell lysate, Lane 3: THP-1 cell lysate, Lane 4: Mouse spleen tissue lysate, Lane 5: Rat spleen tissue lysate, Lane 6: Raw264.7 cell lysate, Lane 7: C6 cell lysate.)

Related Product Information for anti-ACTB antibody
Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR.
Product Categories/Family for anti-ACTB antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
60
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
375
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
NCBI Protein Information
actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN

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Product Notes

The ACTB actb (Catalog #AAA22338) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The beta actin Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's beta actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 12000-1:4000 IHC: 1:200-1:400 IF: 1:200-1:400. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.