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ICC (Immunocytochemistry)
(ICC staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit Actin Monoclonal Antibody | anti-ACTA1 antibody
Actin Antibody
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
Actin; Monoclonal Antibody; Actin Antibody; a actin antibody; ACTA antibody; ACTA1 antibody; Actin alpha skeletal muscle antibody; Actin antibody; actin; alpha 1; skeletal muscle 1 antibody; skeletal muscle antibody; alpha skeletal muscle antibody; actina antibody; actine antibody; ACTS_HUMAN antibody; aktin antibody; Alpha Actin 1 antibody; Alpha skeletal muscle Actin antibody; alpha-actin antibody; Alpha-actin-1 antibody; ASMA antibody; CFTD antibody; CFTD1 antibody; CFTDM antibody; MPFD antibody; NEM1 antibody; NEM2 antibody; NEM3 antibody; nemaline myopathy type 3 antibody; anti-ACTA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Monoclonal
Clone Number
JJ09-29
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-ACTA1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:100-1:500
IHC: 1:50-1:200
ICC: 1:100-1:500
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1, 000 dilution. Positive control: Lane 1:Hybrid fish (crucian-carp) brain tissue Lane 2:Hybrid fish (crucian-carp) kidney tissue )
WB (Western Blot)
(Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1, 000 dilution. Positive control: Lane 1:Hybrid fish (crucian-carp) brain tissue Lane 2:Hybrid fish (crucian-carp) kidney tissue )
WB (Western Blot)
(Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1, 000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: A431 Lane 4: Jurkat)
WB (Western Blot)
(Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1, 000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: A431 Lane 4: Jurkat)
Related Product Information for anti-ACTA1 antibody
All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. alpha-Actin expression is limited to various types of muscle, whereas beta-Actin and gamma-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
Product Categories/Family for anti-ACTA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,051 Da
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin, alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
NCBI Protein Information
actin, alpha skeletal muscle; nemaline myopathy type 3
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Gene Name
ACTA1
UniProt Synonym Gene Names
ACTA
UniProt Entry Name
ACTS_HUMAN
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Product Notes
The ACTA1 acta1 (Catalog #AAA30249) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Actin Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's Actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ACTA1 acta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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