Reactivity
Rabbit
Specificity
No cross-reaction with other factors.
Sequence Length
194
Assay Type
Sandwich
Detection Range
1000 pg/mL-15.6 pg/mL
Sensitivity
Up to 5 pg/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for NGF elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rabbit NGF monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,903 Da
NCBI Official Full Name
nerve growth factor
NCBI Official Synonym Full Names
nerve growth factor (beta polypeptide)
NCBI Official Symbol
ngf
NCBI Official Synonym Symbols
ngfb; ns:zft386; xx:zft386; zgc:109730
NCBI Protein Information
nerve growth factor; zNGF
UniProt Protein Name
Nerve growth factor
UniProt Gene Name
ngf
UniProt Synonym Gene Names
ngfb; NGF; zNGF
UniProt Entry Name
NGF_DANRE