Monkey NGF ELISA Kit | NGF elisa kit
Monkey NGF (Nerve Growth Factor) ELISA Kit
Gene Names
NGF; NGFB; HSAN5; Beta-NGF
Reactivity
Monkey
Synonyms
NGF; N/A; Monkey NGF (Nerve Growth Factor) ELISA Kit; NGF elisa kit
Reactivity
Monkey
Specificity
This kit recognizes Monkey NGF in samples. No significant cross-reactivity or interference between Monkey NGF and analogues was observed.
Sequence Length
299
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
18.75pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Monkey NGF were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Monkey NGF were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Related Product Information for NGF elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey NGF concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey NGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Monkey NGF. You can calculate the concentration of Monkey NGF in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey NGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Monkey NGF. You can calculate the concentration of Monkey NGF in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26,959 Da
NCBI Official Full Name
NGF protein, partial
NCBI Official Synonym Full Names
nerve growth factor (beta polypeptide)
NCBI Official Symbol
NGF
NCBI Official Synonym Symbols
NGFB; HSAN5; Beta-NGF
NCBI Protein Information
beta-nerve growth factor; nerve growth factor, beta subunit
UniProt Protein Name
Beta-nerve growth factor
UniProt Gene Name
NGF
UniProt Synonym Gene Names
NGFB; Beta-NGF
UniProt Entry Name
NGF_HUMAN
