Canine Thrombospondin 1 ELISA Kit | TSP-1 elisa kit
Canine thrombospondin 1 (TSP-1) ELISA Kit
Gene Names
THBS1; TSP; THBS; TSP1; TSP-1; THBS-1
Reactivity
Canine
Synonyms
Thrombospondin 1; N/A; Canine thrombospondin 1 (TSP-1) ELISA Kit; TSP-1 elisa kit
Reactivity
Canine
Specificity
No cross-reaction with other factors.
Sequence Length
1170
Samples
Canine serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
200 ng/mL-3.12 ng/mL
Sensitivity
Up to 0.6 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for TSP-1 elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Canine TSP-1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120,148 Da
NCBI Official Full Name
thrombospondin-1
NCBI Official Synonym Full Names
thrombospondin 1
NCBI Official Symbol
THBS1
NCBI Official Synonym Symbols
TSP; THBS; TSP1; TSP-1; THBS-1
NCBI Protein Information
thrombospondin-1
UniProt Protein Name
Thrombospondin-1
UniProt Gene Name
THBS1
UniProt Synonym Gene Names
TSP; TSP1
UniProt Entry Name
TSP1_HUMAN
