Rat TSP-1 ELISA Kit | TSP-1 elisa kit
Rat TSP-1(Thrombospondin 1)ELISA Kit
Gene Names
THBS1; TSP; THBS; TSP1; TSP-1; THBS-1
Reactivity
Rat
Synonyms
TSP-1; N/A; Rat TSP-1(Thrombospondin 1)ELISA Kit; TSP-1 elisa kit
Reactivity
Rat
Specificity
This kit recognizes natural and recombinant Rat TSP-1. No significant cross-reactivity or interference between Rat TSP-1 and analogues was observed.
Sequence Length
1170
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Related Product Information for TSP-1 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat TSP-1 concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TSP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TSP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TSP-1. You can calculate the concentration of TSP-1 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TSP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TSP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TSP-1. You can calculate the concentration of TSP-1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120,148 Da
NCBI Official Full Name
thrombospondin-1
NCBI Official Synonym Full Names
thrombospondin 1
NCBI Official Symbol
THBS1
NCBI Official Synonym Symbols
TSP; THBS; TSP1; TSP-1; THBS-1
NCBI Protein Information
thrombospondin-1; thrombospondin-1p180
UniProt Protein Name
Thrombospondin-1
UniProt Gene Name
THBS1
UniProt Synonym Gene Names
TSP; TSP1
UniProt Entry Name
TSP1_HUMAN
