Porcine Tight junction protein 1 ELISA Kit | ZO1 elisa kit
Porcine tight junction protein 1 (ZO1) ELISA Kit
Gene Names
TJP2; ZO2; X104; PFIC4; DFNA51; DUP9q21.11; C9DUPq21.11
Reactivity
Porcine
Synonyms
Tight junction protein 1; N/A; Porcine tight junction protein 1 (ZO1) ELISA Kit; ZO1 elisa kit
Reactivity
Porcine
Sequence Length
1190
Assay Type
Sandwich
Detection Range
100 ng/mL-1.56 ng/mL
Sensitivity
Up to 0.5 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for ZO1 elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Porcine ZO1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
137,342 Da
NCBI Official Full Name
tight junction protein ZO-2 isoform 1
NCBI Official Synonym Full Names
tight junction protein 2
NCBI Official Symbol
TJP2
NCBI Official Synonym Symbols
ZO2; X104; PFIC4; DFNA51; DUP9q21.11; C9DUPq21.11
NCBI Protein Information
tight junction protein ZO-2
UniProt Protein Name
Tight junction protein ZO-2
UniProt Gene Name
TJP2
UniProt Synonym Gene Names
X104; ZO2
UniProt Entry Name
ZO2_HUMAN
