General Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit | NAD elisa kit
General Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit
Reactivity
General
Synonyms
Nicotinamide Adenine Dinucleotide (NAD); N/A; General Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit; NAD elisa kit
Reactivity
General
Specificity
This assay has high sensitivity and excellent specificity for detection of NAD. No significant cross-reactivity or interference between NAD and analogues was observed.
Sequence Length
298
Assay Type
Quantitative Competitive
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
123.5-10,000ng/mL
Sensitivity
< 49.3ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NAD were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NAD were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for NAD elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to NAD has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled NAD and unlabeled NAD (Standards or samples) with the pre-coated antibody specific to NAD. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NAD in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NAD in the sample.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NAD in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NAD in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
32,576 Da
NCBI Official Full Name
nicotinamide adenine dinucleotide, partial (mitochondrion)
UniProt Protein Name
NADH-ubiquinone oxidoreductase chain 2
UniProt Gene Name
NADH2
