Human nicotinamide phosphoribosyltransferase ELISA Kit | NAMPT elisa kit
Human visfatin ELISA Kit
Gene Names
NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
Reactivity
Human
Synonyms
nicotinamide phosphoribosyltransferase; N/A; Human visfatin ELISA Kit; 1110035O14Rik; DKFZp666B131; MGC117256; PBEF; PBEF1; VF; VISFATIN; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; NAMPT elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human visfatin. No significant cross-reactivity or interference between human visfatin and analogues was observed.
Sequence Length
491
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, urine, tissue homogenates
Detection Range
0.625 ng/ml-40 ng/ml
Sensitivity
< 0.156 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for NAMPT elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for visfatin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any visfatin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for visfatin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of visfatin bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,521 Da
NCBI Official Full Name
nicotinamide phosphoribosyltransferase
NCBI Official Synonym Full Names
nicotinamide phosphoribosyltransferase
NCBI Official Symbol
NAMPT
NCBI Official Synonym Symbols
VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
NCBI Protein Information
nicotinamide phosphoribosyltransferase; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1
UniProt Protein Name
Nicotinamide phosphoribosyltransferase
UniProt Gene Name
NAMPT
UniProt Synonym Gene Names
PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
UniProt Entry Name
NAMPT_HUMAN
