Human Autophagy Related Protein 5 (ATG5) ELISA Kit | ATG5 elisa kit
Human Autophagy Related Protein 5 (ATG5) ELISA Kit
Reactivity
Human
Synonyms
Autophagy Related Protein 5 (ATG5); N/A; Human Autophagy Related Protein 5 (ATG5) ELISA Kit; ASP; APG5; APG5L; hAPG5; Apoptosis-specific protein; Autophagy protein 5; ATG5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ATG5. No significant cross-reactivity or interference between ATG5 and analogues was observed.
Sequence Length
364
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.78-50ng/mL
Sensitivity
< 0.28ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATG5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATG5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for ATG5 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ATG5 in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ATG5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ATG5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATG5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATG5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ATG5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ATG5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATG5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATG5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,687 Da
NCBI Official Full Name
Autophagy-related protein 5
NCBI Official Symbol
NCU04662
NCBI Protein Information
autophagy protein 5
UniProt Protein Name
Autophagy-related protein 5
UniProt Gene Name
apg-4
UniProt Synonym Gene Names
atg5
