Human Autophagy related protein 13 (KIAA0652) ELISA Kit | KIAA0652 elisa kit

Human Autophagy related protein 13 (KIAA0652) ELISA Kit

Cat. Num. AAA27284

• Gene Names: ATG13; KIAA0652; PARATARG8
• Reactivity: Human
• Synonyms: Autophagy related protein 13 (KIAA0652); N/A; Human Autophagy related protein 13 (KIAA0652) ELISA Kit; KIAA0652 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATG13. No significant cross-reactivity or interference between ATG13 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATG13 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for KIAA0652 elisa kit

Principle of the Assay: ATG13 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATG13 antibody and an ATG13-HRP conjugate. The assay sample and buffer are incubated together with ATG13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG13 concentration since ATG13 from samples and ATG13-HRP conjugate compete for the anti-ATG13 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG13 from the sample, fewer sites are left to bind ATG13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG13 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ATG13 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATG13. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for KIAA0652 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 326806956
• NCBI GeneID: 9776
• NCBI Accession #: NP_001192050.1
• NCBI GenBank Nucleotide #: NM_001205121.1
• Molecular Weight: 60,155 Da
• NCBI Official Full Name: autophagy-related protein 13 isoform 2
• NCBI Official Synonym Full Names: autophagy related 13
• NCBI Official Symbol: ATG13
• NCBI Official Synonym Symbols: KIAA0652; PARATARG8
• NCBI Protein Information: autophagy-related protein 13; ATG13 autophagy related 13 homolog
• UniProt Protein Name: Autophagy-related protein 13
• UniProt Gene Name: ATG13
• UniProt Synonym Gene Names: KIAA0652
• UniProt Entry Name: ATG13_HUMAN