Mouse High mobility group protein B1 ELISA Kit | Hmgb1 elisa kit
Mouse High mobility group protein B1 ELISA Kit
Gene Names
Hmgb1; p30; Hmg1; HMG-1; SBP-1
Reactivity
Mouse
Synonyms
High mobility group protein B1; N/A; Mouse High mobility group protein B1 ELISA Kit; High mobility group protein 1; HMG-1; Hmgb1; Hmg-1; Hmg1; Hmgb1 elisa kit
Reactivity
Mouse
Specificity
Recombinant and natural Mouse High mobility group protein B1
Sequence Length
215
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
0.156 ng/mL -10 ng/mL
Intra-assay Precision
Intra-Assay CV: <=7.1%
Inter-assay Precision
Inter-Assay CV: <=9.2%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Related Product Information for Hmgb1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,894 Da
NCBI Official Full Name
high mobility group protein B1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
Hmgb1
NCBI Official Synonym Symbols
p30; Hmg1; HMG-1; SBP-1
NCBI Protein Information
high mobility group protein B1
UniProt Protein Name
High mobility group protein B1
UniProt Gene Name
Hmgb1
UniProt Synonym Gene Names
Hmg-1; Hmg1; HMG-1
UniProt Entry Name
HMGB1_MOUSE
