Bovine Vascular endothelial growth factor A ELISA Kit | VEGFA elisa kit
Bovine Vascular endothelial growth factor A ELISA Kit
Gene Names
VEGFA; VPF; VEGF; VEGF-A; eVEGF120; eVEGF164
Reactivity
Bovine
Synonyms
Vascular endothelial growth factor A; N/A; Bovine Vascular endothelial growth factor A ELISA Kit; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF; VEGFA elisa kit
Reactivity
Bovine
Sequence Length
190
Samples
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Related Product Information for VEGFA elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,086 Da
NCBI Official Full Name
vascular endothelial growth factor A isoform VEGF-A
NCBI Official Symbol
VEGFA
NCBI Official Synonym Symbols
VPF; VEGF; VEGF-A; eVEGF120; eVEGF164
NCBI Protein Information
vascular endothelial growth factor A
UniProt Protein Name
Vascular endothelial growth factor A
UniProt Gene Name
VEGFA
UniProt Synonym Gene Names
VEGF; VEGF-A; VPF
UniProt Entry Name
VEGFA_BOVIN