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WB (Western Blot)
(WB Suggested Anti-DLD Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysateDLD is supported by BioGPS gene expression data to be expressed in Jurkat)
Rabbit DLD Polyclonal Antibody | anti-DLD antibody
DLD antibody - middle region
Gene Names
DLD; E3; LAD; DLDD; DLDH; GCSL; PHE3
Reactivity
Tested Speciea: Human, RatPredicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
DLD; Polyclonal Antibody; DLD antibody - middle region; anti-DLD antibody
Host
Rabbit
Reactivity
Tested Speciea: Human, Rat
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5mg/mL (varies by lot)
Sequence
Synthetic peptide located within the following region: AGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF
Sequence Length
509
Applicable Applications for anti-DLD antibody
Immunohistochemistry (IHC), Western Blot (WB)
Protein Size (#AA)
509 amino acids
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human DLD
Protein Interactions
HUWE1; FUS; UBC; SUMO1; NEDD8; MDM2; ASB15; PEPD; LDHA; CKB; ASS1; CNDP2; TNFAIP8; DPP3; RPL23; FN1; ATF2; MRPL11; UBAP2; MRPL37; MRPL13; MRPL42; GGH; UQCRC2; UQCRC1; MRPL23; NONO; NDUFB5; NDUFA9; ETFB; DLAT; DBT; AK2; SIRT7; SLC2A4; PSMD4; OGDH; DLD; PDH
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(WB Suggested Anti-DLD Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysateDLD is supported by BioGPS gene expression data to be expressed in Jurkat)
WB (Western Blot)
(WB Suggested Anti-DLD Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysateDLD is supported by BioGPS gene expression data to be expressed in Jurkat)
WB (Western Blot)
(Host: RabbitTarget Name: SERPINA3Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: SERPINA3Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: NOP56Sample Type: MCF7Antibody Dilution: 1.0ug/mlDLD is supported by BioGPS gene expression data to be expressed in MCF7)
WB (Western Blot)
(Host: RabbitTarget Name: NOP56Sample Type: MCF7Antibody Dilution: 1.0ug/mlDLD is supported by BioGPS gene expression data to be expressed in MCF7)
WB (Western Blot)
(Host: RabbitTarget Name: EGFL8Sample Type: HelaAntibody Dilution: 1.0ug/mlDLD is supported by BioGPS gene expression data to be expressed in HeLa)
WB (Western Blot)
(Host: RabbitTarget Name: EGFL8Sample Type: HelaAntibody Dilution: 1.0ug/mlDLD is supported by BioGPS gene expression data to be expressed in HeLa)
WB (Western Blot)
(Host: RabbitTarget Name: DLDSample Tissue: Human Fetal KidneyAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: DLDSample Tissue: Human Fetal KidneyAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(DLD antibody - middle region validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.)
WB (Western Blot)
(DLD antibody - middle region validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.)
IHC (Immunohistochemistry)
(Rabbit Anti-DLD AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Pineal TissueObserved Staining: Cytoplasmic in cell bodies and processes of pinealocytesPrimary Antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
IHC (Immunohistochemistry)
(Rabbit Anti-DLD AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Pineal TissueObserved Staining: Cytoplasmic in cell bodies and processes of pinealocytesPrimary Antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
Related Product Information for anti-DLD antibody
This is a rabbit polyclonal antibody against DLD. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: DLD is the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Target Description: DLD is the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Product Categories/Family for anti-DLD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56kDa
NCBI Official Full Name
dihydrolipoyl dehydrogenase, mitochondrial isoform 1
NCBI Official Synonym Full Names
dihydrolipoamide dehydrogenase
NCBI Official Symbol
DLD
NCBI Official Synonym Symbols
E3; LAD; DLDD; DLDH; GCSL; PHE3
NCBI Protein Information
dihydrolipoyl dehydrogenase, mitochondrial
Similar Products
Product Notes
The DLD (Catalog #AAA23580) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DLD antibody - middle region reacts with Tested Speciea: Human, Rat Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's DLD can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the DLD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: AGEMVNEAAL ALEYGASCED IARVCHAHPT LSEAFREANL AASFGKSINF. It is sometimes possible for the material contained within the vial of "DLD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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