Mouse anti-Human Estrogen Receptor Alpha Monoclonal Antibody | anti-ESR1 antibody

Mouse anti-Estrogen Receptor Alpha Monoclonal Antibody [119-13]

Cat. Num. AAA23783

• Gene Names: ESR1; ER; ESR; Era; ESRA; NR3A1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: Estrogen Receptor Alpha, Antibody; Mouse anti-Estrogen Receptor Alpha Monoclonal Antibody [119-13]; estrogen nuclear receptor alpha; ESTRR; NR3A1; oestrogen receptor alpha; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor; nuclear receptor subfamily 3 group A member 1; ESRA; ESR; ER-alpha; Era; ER; E2 receptor alpha; estradiol receptor; Estrogen receptor; estrogen receptor 1; anti-ESR1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: 119-13
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-ESR1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 6 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
• Immunogen: Recombinant human Estrogen Receptor Alpha
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human Estrogen Receptor alpha by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Mouse anti-Estrogen Receptor alpha monoclonal antibody [119-13] (AAA23783 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation)

(Detection of human Estrogen Receptor alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF7 cells prepared using NETN lysis buffer. Antibodies: Mouse anti-Estrogen Receptor alpha monoclonal antibody [119-13] (AAA23783 lot 1) used for IP at 6 ug/mg lysate. Estrogen Receptor alpha was also immunoprecipitated by another anti-Estrogen Receptor alpha antibody against a differnet epitope . For blotting immunoprecipitated Estrogen Receptor alpha, A500-026 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IHC (Immunohistochemistry)

(Detection of human Estrogen Receptor Alpha in FFPE breast carcinoma by immunohistochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human Estrogen Receptor Alpha in FFPE breast carcinoma by immunohistochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human Estrogen Receptor Alpha in FFPE MCF7 cells by immunocytochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human Estrogen Receptor Alpha (shaded) in MCF7 cells by flow cytometry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783) or secondary antibody only (unshaded). Secondary: DyLight 650-conjugated goat anti-mouse IgG .)

Related Product Information for anti-ESR1 antibody

The estrogen receptor (ER) is the steroid-hormone nuclear receptor that binds estrogen and functions to activate genes involved in a variety of biological processes. Two forms of the ER, ER alpha and ER beta, are encoded by independent genes, and each plays different roles in gene regulation. Additional variants of ER beta have also been reported; these include ERb2, ERb3, ERb4, and ERb5. All forms of the ER have the ability to heterodimerize with each other. Differences in localization and concentrations as well as heterodimerization among the variants may serve to achieve distinct cell and tissue responses. ER alpha generally functions as an activator of transcription while ER beta functions as a repressor.

Product Categories/Family for anti-ESR1 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 62821794
• NCBI GeneID: 2099
• NCBI Accession #: NP_000116.2
• NCBI GenBank Nucleotide #: NM_000125.3
• UniProt Accession #: P03372; Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7
• Molecular Weight: 66,216 Da
• NCBI Official Full Name: estrogen receptor
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: ESR1
• NCBI Official Synonym Symbols: ER; ESR; Era; ESRA; NR3A1
• NCBI Protein Information: estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha delta 4 +49 isoform; nuclear receptor subfamily 3 group A member 1; estrogen
• UniProt Protein Name: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER
• UniProt Entry Name: ESR1_HUMAN