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WB (Western Blot)
(Detection of human Estrogen Receptor alpha by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Mouse anti-Estrogen Receptor alpha monoclonal antibody [119-13] (AAA23783 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
Mouse anti-Human Estrogen Receptor Alpha Monoclonal Antibody | anti-ESR1 antibody
Mouse anti-Estrogen Receptor Alpha Monoclonal Antibody [119-13]
Gene Names
ESR1; ER; ESR; Era; ESRA; NR3A1
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
Estrogen Receptor Alpha; Monoclonal Antibody; Mouse anti-Estrogen Receptor Alpha Monoclonal Antibody [119-13]; estrogen nuclear receptor alpha; ESTRR; NR3A1; oestrogen receptor alpha; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor; nuclear receptor subfamily 3 group A member 1; ESRA; ESR; ER-alpha; Era; ER; E2 receptor alpha; estradiol receptor; Estrogen receptor; estrogen receptor 1; anti-ESR1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
119-13
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ESR1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
Immunogen
Recombinant human Estrogen Receptor Alpha
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Estrogen Receptor alpha by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Mouse anti-Estrogen Receptor alpha monoclonal antibody [119-13] (AAA23783 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human Estrogen Receptor alpha by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Mouse anti-Estrogen Receptor alpha monoclonal antibody [119-13] (AAA23783 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human Estrogen Receptor alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF7 cells prepared using NETN lysis buffer. Antibodies: Mouse anti-Estrogen Receptor alpha monoclonal antibody [119-13] (AAA23783 lot 1) used for IP at 6 ug/mg lysate. Estrogen Receptor alpha was also immunoprecipitated by another anti-Estrogen Receptor alpha antibody against a differnet epitope . For blotting immunoprecipitated Estrogen Receptor alpha, A500-026 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Estrogen Receptor alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF7 cells prepared using NETN lysis buffer. Antibodies: Mouse anti-Estrogen Receptor alpha monoclonal antibody [119-13] (AAA23783 lot 1) used for IP at 6 ug/mg lysate. Estrogen Receptor alpha was also immunoprecipitated by another anti-Estrogen Receptor alpha antibody against a differnet epitope . For blotting immunoprecipitated Estrogen Receptor alpha, A500-026 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human Estrogen Receptor Alpha in FFPE breast carcinoma by immunohistochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human Estrogen Receptor Alpha in FFPE breast carcinoma by immunohistochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human Estrogen Receptor Alpha in FFPE breast carcinoma by immunohistochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human Estrogen Receptor Alpha in FFPE breast carcinoma by immunohistochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Estrogen Receptor Alpha in FFPE MCF7 cells by immunocytochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Estrogen Receptor Alpha in FFPE MCF7 cells by immunocytochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human Estrogen Receptor Alpha (shaded) in MCF7 cells by flow cytometry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783) or secondary antibody only (unshaded). Secondary: DyLight 650-conjugated goat anti-mouse IgG .)
FCM (Flow Cytometry)
(Detection of human Estrogen Receptor Alpha (shaded) in MCF7 cells by flow cytometry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783) or secondary antibody only (unshaded). Secondary: DyLight 650-conjugated goat anti-mouse IgG .)
Related Product Information for anti-ESR1 antibody
The estrogen receptor (ER) is the steroid-hormone nuclear receptor that binds estrogen and functions to activate genes involved in a variety of biological processes. Two forms of the ER, ER alpha and ER beta, are encoded by independent genes, and each plays different roles in gene regulation. Additional variants of ER beta have also been reported; these include ERb2, ERb3, ERb4, and ERb5. All forms of the ER have the ability to heterodimerize with each other. Differences in localization and concentrations as well as heterodimerization among the variants may serve to achieve distinct cell and tissue responses. ER alpha generally functions as an activator of transcription while ER beta functions as a repressor.
Product Categories/Family for anti-ESR1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,216 Da
NCBI Official Full Name
estrogen receptor
NCBI Official Synonym Full Names
estrogen receptor 1
NCBI Official Symbol
ESR1
NCBI Official Synonym Symbols
ER; ESR; Era; ESRA; NR3A1
NCBI Protein Information
estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha delta 4 +49 isoform; nuclear receptor subfamily 3 group A member 1; estrogen
UniProt Protein Name
Estrogen receptor
UniProt Gene Name
ESR1
UniProt Synonym Gene Names
ESR; NR3A1; ER
UniProt Entry Name
ESR1_HUMAN
Similar Products
Product Notes
The ESR1 esr1 (Catalog #AAA23783) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Estrogen Receptor Alpha Monoclonal Antibody [119-13] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Estrogen Receptor Alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 6 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the ESR1 esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Estrogen Receptor Alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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