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WB (Western Blot)
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WB (Western Blot) (Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)

gamma-H2AX recombinant antibody

Rabbit anti-gamma-H2AX Recombinant Monoclonal Antibody [BLR053F]

Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
gamma-H2AX; Monoclonal Recombinant Antibody; Rabbit anti-gamma-H2AX Recombinant Monoclonal Antibody [BLR053F]; H2AX histone; histone H2AX; H2AX; H2A/X; H2A.X; histone H2A.x; Histone H2AX; H2A histone family member X; gamma-H2AX recombinant antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR053F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for gamma-H2AX recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Surrounding Serine 140
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)

WB (Western Blot) (Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)

IP (Immunoprecipitation)

(Detection of human Gamma-H2AX by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM etoposide or mock treated (-). Antibodies: Rabbit anti-Gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used for IP at 20 ul/mg lysate. H2AX was also immunoprecipitated by goat anti-H2AX antibody . For blotting immunoprecipitated Gamma-H2AX, AAA23813 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: goat anti-H2AX antibody .)

IP (Immunoprecipitation) (Detection of human Gamma-H2AX by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM etoposide or mock treated (-). Antibodies: Rabbit anti-Gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used for IP at 20 ul/mg lysate. H2AX was also immunoprecipitated by goat anti-H2AX antibody . For blotting immunoprecipitated Gamma-H2AX, AAA23813 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: goat anti-H2AX antibody .)

IHC (Immunohistochemistry)

(Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813) used at 1:100. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI.)

IHC (Immunohistochemistry) (Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813) used at 1:100. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI.)

IHC (Immunohistochemistry)

(Detection of mouse gamma-H2AX by immunohistochemistry. Sample: FFPE section of mouse CT26 tumor. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.)

IHC (Immunohistochemistry) (Detection of mouse gamma-H2AX by immunohistochemistry. Sample: FFPE section of mouse CT26 tumor. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.)

IHC (Immunohistochemistry)

(Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human basal cell carcinoma. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.)

IHC (Immunohistochemistry) (Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human basal cell carcinoma. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.)

ICC (Immunocytochemistry)

(Detection of human gamma-H2AX by immunocytochemistry. Sample: FFPE section of human HeLa cells treated with etoposide. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.)

ICC (Immunocytochemistry) (Detection of human gamma-H2AX by immunocytochemistry. Sample: FFPE section of human HeLa cells treated with etoposide. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.)

FCM (Flow Cytometry)

(Detection of human gamma H2AX (shaded) in etoposide treated HEK293T cells by flow cytometry. Antibody: Rabbit anti-gamma H2AX recombinant monoclonal (AAA23813) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human gamma H2AX (shaded) in etoposide treated HEK293T cells by flow cytometry. Antibody: Rabbit anti-gamma H2AX recombinant monoclonal (AAA23813) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for gamma-H2AX recombinant antibody
H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. Gamma H2AX is the phosphorylated form of H2AX that results in response to DNA damage.
Product Categories/Family for gamma-H2AX recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,145 Da
NCBI Official Full Name
histone H2A.x
NCBI Official Synonym Full Names
H2A histone family, member X
NCBI Official Symbol
H2AFX
NCBI Official Synonym Symbols
H2AX; H2A.X; H2A/X
NCBI Protein Information
histone H2A.x; H2AX histone
UniProt Protein Name
Histone H2A.x
UniProt Gene Name
H2AFX
UniProt Synonym Gene Names
H2AX; H2a/x
UniProt Entry Name
H2AX_HUMAN

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Product Notes

The gamma-H2AX h2afx (Catalog #AAA23813) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-gamma-H2AX Recombinant Monoclonal Antibody [BLR053F] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's gamma-H2AX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the gamma-H2AX h2afx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "gamma-H2AX, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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