beta Catenin Recombinant Antibody | CTNNB1 recombinant antibody

Rabbit anti-beta Catenin Recombinant Monoclonal Antibody [BLR086G]

Cat. Num. AAA23827

• Gene Names: CTNNB1; CTNNB; MRD19; armadillo
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: beta Catenin, Recombinant Antibody; Rabbit anti-beta Catenin Recombinant Monoclonal Antibody [BLR086G]; armadillo; MRD19; EVR7; CTNNB; catenin beta-1; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; Catenin beta-1; catenin beta 1; CTNNB1 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR086G
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 250ug/ml (varies by lot)

• Applicable Applications for CTNNB1 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 20 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
• Immunogen: Between 731 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human and mouse beta-Catenin by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, LNCaP, K-562, GaMG, Jurkat, A-549, MCF-7, HeLa, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-beta-Catenin recombinant monoclonal antibody (AAA23827 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)

IP (Immunoprecipitation)

(Detection of human beta Catenin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1) used for IP at 20 ul per reaction. beta Catenin was also immunoprecipitated by rabbit anti-beta Catenin antibodies . For blotting immunoprecipitated beta Catenin, AAA23827 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IP (Immunoprecipitation)

(Detection of human beta Catenin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1) used for IP at 20 ul per reaction. beta Catenin was also immunoprecipitated by rabbit anti-beta Catenin antibodies . For blotting immunoprecipitated beta Catenin, AAA23827 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistchemistry)

(Detection of human beta Catenin in FFPE prostate carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)

IHC (Immunohistochemistry)

(Detection of mouse beta Catenin in FFPE plasmacytoma by IHC. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human beta Catenin in FFPE colon carcinoma by IHC. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human beta Catenin in FFPE OVCAR-3 cells by ICC. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human beta Catenin (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of mouse beta Catenin (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for CTNNB1 recombinant antibody

beta Catenin plays an integral role in the Wnt signaling pathway and is critical to morphogenic signaling and body axis specification. Beta Catenin exists in complexes with cadherins, transmembrane proteins that mediate cell adhesion. When Wnt-signaling proteins bind their receptors, beta Catenin is stabilized and accumulates in the cell. Translocation of stabilized beta Catenin allows its association with transcription factors and the activation of specific gene expression.

Product Categories/Family for CTNNB1 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4503131
• NCBI GeneID: 1499
• NCBI Accession #: NP_001895.1
• NCBI GenBank Nucleotide #: NM_001904.3
• UniProt Accession #: P35222; Q8NEW9; Q8NI94; Q9H391; A8K1L7
• Molecular Weight: 9,501 Da
• NCBI Official Full Name: catenin beta-1
• NCBI Official Synonym Full Names: catenin (cadherin-associated protein), beta 1, 88kDa
• NCBI Official Symbol: CTNNB1
• NCBI Official Synonym Symbols: CTNNB; MRD19; armadillo
• NCBI Protein Information: catenin beta-1
• UniProt Protein Name: Catenin beta-1
• UniProt Gene Name: CTNNB1
• UniProt Synonym Gene Names: CTNNB
• UniProt Entry Name: CTNB1_HUMAN