Standard Curve (Sample)
Mouse Estrogen Receptor beta, ER-beta ELISA Kit | ER-beta elisa kit
Mouse Estrogen Receptor beta, ER-beta ELISA Kit
Standard Curve (Sample)
Standard Curve (Sample)
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Estrogen Receptor beta (also known as ER-beta) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
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Product Notes
The Mouse ER-beta esr2 (Catalog #AAA18951) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA18951 ELISA Kit recognizes Mouse ER-beta. It is sometimes possible for the material contained within the vial of "Estrogen Receptor beta, ER-beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
Is this estrogen receptor reagent specific to mouse ERα, ERβ, or both?
This AAA Biotech reagent specifically targets mouse estrogen receptor beta (ER-beta/ERβ/ESR2). The kit is designed for the selective detection of ER-beta in mouse samples, distinguished from the alpha isoform. This specificity is critical since ERα and ERβ have distinct tissue distributions and biological functions.
What applications is the mouse ER antibody/protein suitable for (IHC, WB, IF)?
Mouse ER-beta antibodies are validated for multiple applications, including immunohistochemistry (IHC), Western blot (WB), and immunofluorescence (IF). Each method employs different dilutions and protocols. Immunohistochemistry commonly uses 1:100 dilution, while specific dilutions vary based on tissue type, sample preparation, and detection methodology employed.
Does it cross-react with human or rat estrogen receptors?
Mouse ER-beta antibodies typically show limited cross-reactivity with human or rat estrogen receptors due to species-specific sequence differences. While some antibodies demonstrate broad species reactivity, this reagent is optimized specifically for mouse ER-beta detection. Cross-reactivity testing is recommended when using non-mouse samples to ensure accurate results.
What sample types or tissues work best for detecting mouse ER?
Mouse ER-beta is optimally detected in reproductive tissues, including ovaries, uterus, and oviduct, which show high ER-beta expression. Serum, plasma, and tissue homogenates are appropriate sample types for this ELISA kit. Non-reproductive tissues like the brain show variable expression depending on developmental stage and hormone conditions.
What dilution or concentration is recommended for optimal results?
Specific dilution recommendations depend on the application. For ELISA sandwich kits, concentrated antibodies typically require dilution before use (100× concentrated format is standard). Specific optimal dilutions should be referenced in the AAA Biotech product documentation provided with your kit for precise application guidelines.
How should the mouse estrogen receptor reagent be stored to maintain stability?
Store the mouse estrogen receptor reagent at 2-8°C (refrigeration temperature). The product remains stable until the expiration date printed on the label when stored under these conditions. Do not freeze undiluted reagents. Diluted working solutions should be used promptly, and remaining portions should be stored at 2-8°C immediately after use.
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