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WB (Western Blot)
(Detection of human T-bet/TBX21 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, HEK293T, MJ/G11, HeLa, Jurkat, NK-92, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal .)
T-bet/TBX21 recombinant antibody
Rabbit anti-T-bet/TBX21 Recombinant Monoclonal Antibody [BLR110H]
Gene Names
TBX21; TBET; T-PET; T-bet; TBLYM
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
T-bet/TBX21; Monoclonal Recombinant Antibody; Rabbit anti-T-bet/TBX21 Recombinant Monoclonal Antibody [BLR110H]; T-box transcription factor TBX21; T-PET; transcription factor TBLYM; T-cell-specific T-box transcription factor T-bet; T-box expressed in T cells; TBLYM; TBET; T-bet; T-box protein 21; T-box 21; T-bet/TBX21 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR110H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for T-bet/TBX21 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed and permeabilized cells were stained with 0.05 ul per 1 x 10^6 cells.
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed and permeabilized cells were stained with 0.05 ul per 1 x 10^6 cells.
Immunogen
Between 485 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human T-bet/TBX21 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, HEK293T, MJ/G11, HeLa, Jurkat, NK-92, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal .)
WB (Western Blot)
(Detection of human T-bet/TBX21 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, HEK293T, MJ/G11, HeLa, Jurkat, NK-92, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal .)
IP (Immunoprecipitation)
(Detection of human T-bet/TBX21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ/G11 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1) used for IP at 6 ul per reaction. T-bet/TBX21 was also immunoprecipitated by rabbit anti-T-bet/TBX21 antibody (BL-1505B-4G6). For blotting immunoprecipitated T-bet/TBX21, AAA23837 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human T-bet/TBX21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ/G11 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1) used for IP at 6 ul per reaction. T-bet/TBX21 was also immunoprecipitated by rabbit anti-T-bet/TBX21 antibody (BL-1505B-4G6). For blotting immunoprecipitated T-bet/TBX21, AAA23837 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human T-bet/TBX21 in FFPE Hodgkin's lymphoma by IHC. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human T-bet/TBX21 in FFPE Hodgkin's lymphoma by IHC. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human T-bet/TBX21 in FFPE seminoma by IHC. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human T-bet/TBX21 in FFPE seminoma by IHC. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human T-bet/TBX21 in FFPE MJ cells by ICC. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human T-bet/TBX21 in FFPE MJ cells by ICC. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human T-Bet/TBX21 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-T-Bet/TBX21 recombinant monoclonal (AAA23837 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human T-Bet/TBX21 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-T-Bet/TBX21 recombinant monoclonal (AAA23837 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for T-bet/TBX21 recombinant antibody
T-bet/TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. TBX21 is a lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and activating transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. [taken from NCBI Entrez Gene (Gene ID: 30009)].
Product Categories/Family for T-bet/TBX21 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58,328 Da
NCBI Official Full Name
T-box transcription factor TBX21
NCBI Official Synonym Full Names
T-box 21
NCBI Official Symbol
TBX21
NCBI Official Synonym Symbols
TBET; T-PET; T-bet; TBLYM
NCBI Protein Information
T-box transcription factor TBX21; T-box protein 21; T-box expressed in T cells; transcription factor TBLYM; T-cell-specific T-box transcription factor T-bet
UniProt Protein Name
T-box transcription factor TBX21
UniProt Gene Name
TBX21
UniProt Synonym Gene Names
TBET; TBLYM; T-box protein 21
UniProt Entry Name
TBX21_HUMAN
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Product Notes
The T-bet/TBX21 tbx21 (Catalog #AAA23837) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-T-bet/TBX21 Recombinant Monoclonal Antibody [BLR110H] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's T-bet/TBX21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 6 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed and permeabilized cells were stained with 0.05 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the T-bet/TBX21 tbx21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T-bet/TBX21, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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