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WB (Western Blot)
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WB (Western Blot) (Detection of human BMI1 by western blot. Samples: Whole cell lysate (10 ug) from MOLT-4, HeLa, HEK293T, Jurkat, LNCaP, RKO, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

BMI1 recombinant antibody

Rabbit anti-BMI1 Recombinant Monoclonal Antibody [BLR119H]

Gene Names
BMI1; PCGF4; RNF51; FLVI2/BMI1
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
BMI1; Monoclonal Recombinant Antibody; Rabbit anti-BMI1 Recombinant Monoclonal Antibody [BLR119H]; polycomb complex protein BMI-1; flvi-2/bmi-1; RNF51; ring finger protein 51; polycomb group RING finger protein 4; polycomb group protein Bmi1; murine leukemia viral (bmi-1) oncogene homolog; B lymphoma Mo-MLV insertion region 1 homolog; FLVI2/BMI1; BMI1 polycomb ring finger proto-oncogene; BMI1 polycomb ring finger oncogene; PCGF4; Polycomb complex protein BMI-1; BMI1 proto-oncogene; polycomb ring finger; BMI1 recombinant antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR119H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for BMI1 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
Immunogen
Between 623 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human BMI1 by western blot. Samples: Whole cell lysate (10 ug) from MOLT-4, HeLa, HEK293T, Jurkat, LNCaP, RKO, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot) (Detection of human BMI1 by western blot. Samples: Whole cell lysate (10 ug) from MOLT-4, HeLa, HEK293T, Jurkat, LNCaP, RKO, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot)

(Detection of mouse BMI1 by western blot. Samples: Whole cell lysate (10 ug) from mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot) (Detection of mouse BMI1 by western blot. Samples: Whole cell lysate (10 ug) from mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation)

(Detection of human BMI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1) used for IP at 6 ul/mg lysate. BMI1 was also immunoprecipitated by rabbit anti-BMI1 antibodies and BL1683A-4F6. For blotting immunoprecipitated BMI1, AAA23838 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IP (Immunoprecipitation) (Detection of human BMI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1) used for IP at 6 ul/mg lysate. BMI1 was also immunoprecipitated by rabbit anti-BMI1 antibodies and BL1683A-4F6. For blotting immunoprecipitated BMI1, AAA23838 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry)

(Detection of human BMI1 in FFPE prostate carcinoma by immunohistochemistry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human BMI1 in FFPE prostate carcinoma by immunohistochemistry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of mouse BMI1 in FFPE renal cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of mouse BMI1 in FFPE renal cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human BMI1 in FFPE LNCaP cells by immunocytochemistry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human BMI1 in FFPE LNCaP cells by immunocytochemistry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of mouse BMI1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of mouse BMI1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of human BMI1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human BMI1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for BMI1 recombinant antibody
BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) is a core component of the polycomb group (PcG) multiprotein PRC1 complex which plays an important role in transcriptional repression of gene expression during development. BMI1 association with RING protein 1B is important to the E3 ligase activity of RING1B in the PRC1 complex. BMI1 is an oncogene whose overexpression is associated with many types of human tumors and is a stem-cell marker and regulator of stem cell self-renewal.
Product Categories/Family for BMI1 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
648
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,949 Da
NCBI Official Full Name
polycomb complex protein BMI-1
NCBI Official Synonym Full Names
BMI1 proto-oncogene, polycomb ring finger
NCBI Official Symbol
BMI1
NCBI Official Synonym Symbols
PCGF4; RNF51; FLVI2/BMI1
NCBI Protein Information
polycomb complex protein BMI-1; flvi-2/bmi-1; ring finger protein 51; polycomb group protein Bmi1; BMI1 polycomb ring finger oncogene; polycomb group RING finger protein 4; BMI1 polycomb ring finger proto-oncogene; B lymphoma Mo-MLV insertion region 1 hom
UniProt Protein Name
Polycomb complex protein BMI-1
UniProt Gene Name
BMI1
UniProt Synonym Gene Names
PCGF4; RNF51
UniProt Entry Name
BMI1_HUMAN

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Product Notes

The BMI1 bmi1 (Catalog #AAA23838) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BMI1 Recombinant Monoclonal Antibody [BLR119H] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BMI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 6 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the BMI1 bmi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMI1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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