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WB (Western Blot)
(Detection of human UBQLN4 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from MDA-MB-231 cells (WT and KO). Antibodies: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846 lot 1) used for WB at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody . (KO cells: Jachimowicz et al, Cell, 2019). )
UBQLN4/CIP75/Ubiquilin 4 Recombinant Antibody | UBQLN4/CIP75 recombinant antibody
Rabbit anti-UBQLN4/CIP75/Ubiquilin 4 Recombinant Monoclonal Antibody [BLR145J]
Gene Names
UBQLN4; A1U; A1Up; UBIN; CIP75; C1orf6
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
UBQLN4/CIP75/Ubiquilin 4; Monoclonal Recombinant Antibody; Rabbit anti-UBQLN4/CIP75/Ubiquilin 4 Recombinant Monoclonal Antibody [BLR145J]; ataxin-1 ubiquitin-like-interacting protein A1U; ubiquilin-4; UBIN; connexin43-interacting protein of 75 kDa; C1orf6; ataxin-1 ubiquitin-like interacting protein; ataxin-1 interacting ubiquitin-like protein; A1Up; A1U; CIP75; Ubiquilin-4; ubiquilin 4; UBQLN4/CIP75 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR145J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for UBQLN4/CIP75 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Residues 2-601 (full length)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human UBQLN4 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from MDA-MB-231 cells (WT and KO). Antibodies: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846 lot 1) used for WB at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody . (KO cells: Jachimowicz et al, Cell, 2019). )
WB (Western Blot)
(Detection of human UBQLN4 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from MDA-MB-231 cells (WT and KO). Antibodies: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846 lot 1) used for WB at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody . (KO cells: Jachimowicz et al, Cell, 2019). )
WB (Western Blot)
(Detection of human UBQLN4 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, OVCAR-3, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human UBQLN4 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, OVCAR-3, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human UBQLN4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846 lot 1) used for IP at 20 ul/mg lysate. UBQLN4 was also immunoprecipitated by others rabbit anti-UBQLN4 antibodies . For blotting immunoprecipitated UBQLN4, AAA23846 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human UBQLN4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846 lot 1) used for IP at 20 ul/mg lysate. UBQLN4 was also immunoprecipitated by others rabbit anti-UBQLN4 antibodies . For blotting immunoprecipitated UBQLN4, AAA23846 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human UBQLN4 in FFPE lung carcinoma by immunohistochemistry. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human UBQLN4 in FFPE lung carcinoma by immunohistochemistry. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human UBQLN4 in FFPE Ovcar-4 cells by immunocytochemistry. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human UBQLN4 in FFPE Ovcar-4 cells by immunocytochemistry. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human UBQLN4/CIP75/Ubiquilin 4 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human UBQLN4/CIP75/Ubiquilin 4 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody (AAA23846) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for UBQLN4/CIP75 recombinant antibody
Ubiquilin-4 (UBQLN4) plays a role in the regulation of protein degradation via the ubiquitin-proteasome system (UPS). It mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome. UBQLN4 Plays a role in the regulation of the proteasomal degradation of non-ubiquitinated GJA1 (By similarity). It acts as an adapter protein that recruits UBQLN1 to the autophagy machinery and mediates the association of UBQLN1 with autophagosomes and the autophagy-related protein LC3 (MAP1LC3A/B/C) and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:23459205) [taken from NCBI Entrez Gene (Gene ID: 56893)].
Product Categories/Family for UBQLN4/CIP75 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,853 Da
NCBI Official Full Name
ubiquilin-4
NCBI Official Synonym Full Names
ubiquilin 4
NCBI Official Symbol
UBQLN4
NCBI Official Synonym Symbols
A1U; A1Up; UBIN; CIP75; C1orf6
NCBI Protein Information
ubiquilin-4; connexin43-interacting protein of 75 kDa; ataxin-1 interacting ubiquitin-like protein; ataxin-1 ubiquitin-like interacting protein; ataxin-1 ubiquitin-like-interacting protein A1U
UniProt Protein Name
Ubiquilin-4
UniProt Gene Name
UBQLN4
UniProt Synonym Gene Names
C1orf6; CIP75; UBIN; A1Up; CIP75
UniProt Entry Name
UBQL4_HUMAN
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Product Notes
The UBQLN4/CIP75 ubqln4 (Catalog #AAA23846) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-UBQLN4/CIP75/Ubiquilin 4 Recombinant Monoclonal Antibody [BLR145J] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBQLN4/CIP75/Ubiquilin 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the UBQLN4/CIP75 ubqln4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBQLN4/CIP75/Ubiquilin 4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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