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Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Mesothelin Mouse Monoclonal Antibody (MSLN/2131). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse Mesothelin Monoclonal Antibody | anti-MSLN antibody
Mesothelin (Mesothelial Marker)
Gene Names
MSLN; MPF; SMRP
Reactivity
Human, Mouse, Rat. Others not known.
Applications
Immunohistochemistry
Synonyms
Mesothelin, Antibody; Mesothelin (Mesothelial Marker); CAK1; Megakaryocyte potentiating factor; Mesothelin; MSLN; SMR; SMRP; anti-MSLN antibody
Host
Mouse
Reactivity
Human, Mouse, Rat. Others not known.
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
MSLN/2131
Specificity
Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
622
Applicable Applications for anti-MSLN antibody
Immunohistochemistry (IHC) Formalin
Application Notes
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein
Cellular Localization
Cell Surface and Secreted
Hu-chromosome Location
16p13.3
Positive Control
CAPAN-2 or HeLa cells. Lung Mesothelioma.
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Mesothelin Mouse Monoclonal Antibody (MSLN/2131). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Mesothelin Mouse Monoclonal Antibody (MSLN/2131). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IHC (Immunohistchemistry)
(Formalin-fixed, paraffin-embedded Rat Stomach stained with Mesothelin Mouse Monoclonal Antibody (MSLN/2131).)
IHC (Immunohistchemistry)
(Formalin-fixed, paraffin-embedded Rat Stomach stained with Mesothelin Mouse Monoclonal Antibody (MSLN/2131).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded Mouse Lung stained with Mesothelin Mouse Monoclonal Antibody (MSLN/2131).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded Mouse Lung stained with Mesothelin Mouse Monoclonal Antibody (MSLN/2131).)
SDS-PAGE
(SDS-PAGE Analysis Purified Mesothelin Mouse Monoclonal Antibody (MSLN/2131).Confirmation of Integrity and Purity of the Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified Mesothelin Mouse Monoclonal Antibody (MSLN/2131).Confirmation of Integrity and Purity of the Antibody.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Mesothelin Mouse Monoclonal Antibody (MSLN/2131).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Mesothelin Mouse Monoclonal Antibody (MSLN/2131).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Mesothelin Mouse Monoclonal Antibody (MSLN/2131).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Mesothelin Mouse Monoclonal Antibody (MSLN/2131).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lung Mesothelioma stained with Mesothelin Mouse Monoclonal Antibody (MSLN/2131).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lung Mesothelioma stained with Mesothelin Mouse Monoclonal Antibody (MSLN/2131).)
References
Chang, K. and Pastan, I. 1996. Molecular cloning of Mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40kDa (Mature form); 69kDa (Precursor)
NCBI Official Full Name
mesothelin isoform 1 preproprotein
NCBI Official Synonym Full Names
mesothelin
NCBI Official Symbol
MSLN
NCBI Official Synonym Symbols
MPF; SMRP
NCBI Protein Information
mesothelin
UniProt Protein Name
Mesothelin
UniProt Gene Name
MSLN
UniProt Synonym Gene Names
MPF; MPF
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Product Notes
The MSLN msln (Catalog #AAA23897) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mesothelin (Mesothelial Marker) reacts with Human, Mouse, Rat. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's Mesothelin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin. IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the MSLN msln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mesothelin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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