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SDS-PAGE
(SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Purity and Integrity of Antibody.)
Rabbit CD34 Monoclonal Antibody | anti-CD34 antibody
CD34 (Hematopoietic Stem Cell & Endothelial Marker)
Reactivity
Human, Rat. Others not known.
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
CD34, Antibody; CD34 (Hematopoietic Stem Cell & Endothelial Marker); Hematopoietic Progenitor Cell Antigen; HPCA1; Mucosialin; anti-CD34 antibody
Host
Rabbit
Reactivity
Human, Rat. Others not known.
Clonality
Monoclonal
Isotype
IgG
Clone Number
HPCA1/2598R
Specificity
This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
328
Applicable Applications for anti-CD34 antibody
Immunohistology (Formalin-Fixed)
Application Notes
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
Recombinant full-length human HPCA1 protein
Positive Control
KG-1 Cells. Tonsil, or Angiosarcoma
Cellular Localization
Cell Surface and Cytoplasmic
Chromosome Location
1q32.2
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)
Related Product Information for anti-CD34 antibody
Recombinant Rabbit Monoclonal Antibody
References
Sato T et al. Blood 94:2548-2554 (1999).; Felshow DM et al. Blood 97:3768-3775 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90-110kDa
NCBI Official Full Name
hematopoietic progenitor cell antigen CD34 isoform a
NCBI Official Synonym Full Names
CD34 molecule
NCBI Official Symbol
CD34
NCBI Protein Information
hematopoietic progenitor cell antigen CD34
UniProt Protein Name
Hematopoietic progenitor cell antigen CD34
UniProt Gene Name
CD34
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Product Notes
The CD34 cd34 (Catalog #AAA23910) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD34 (Hematopoietic Stem Cell & Endothelial Marker) reacts with Human, Rat. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's CD34 can be used in a range of immunoassay formats including, but not limited to, Immunohistology (Formalin-Fixed). Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the CD34 cd34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD34, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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