Mouse anti-Human, Mouse S100A4/Metastasin/Calvasculin Antibody | anti-S100A4 antibody

S100A4/Metastasin/Calvasculin (Marker of Tumor Metastasis)

Cat. Num. AAA23934

• Gene Names: S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: S100A4/Metastasin/Calvasculin; S100A4/Metastasin/Calvasculin (Marker of Tumor Metastasis); S100 calcium-binding protein A4; CAPL; Fibroblast specific protein 1 (FSP1); Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Metastasin; Placental calcium-binding protein; anti-S100A4 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG1, kappa
• Clone Number: rS100A4/1481
• Specificity: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 101

• Applicable Applications for anti-S100A4 antibody: Flow Cytometry (FC/FACS), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
• Application Notes: FC/FACS: 1-2ug/million cells; WB: 1-2ug/ml; IF: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HeLa, A549 or A375 cells (IF/FACS). HeLa, A549, T98G or A375 cell lysate (WB). Human placenta tissue (IHC).
• Immunogen: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic and Nuclear
• Hu-chromosome Location: 1q21
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

WB (Western Blot)

(Western Blot Analysis of Panc-1 cell lysate using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).)

SDS-PAGE

(SDS-PAGE Analysis Purified S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481). Confirmation of Purity and Integrity of Antibody.)

IF (Immunofluorescence)

(Immunofluorescence staining of A549 cells using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.)

FCM (Flow Cytometry)

(Flow Cytometric Analysis of A549 cells using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).)

References

Ismail NI, Kaur G, Hashim H, Hassan MS. S100A4 overexpression proves to be independent marker for breast cancer progression. Cancer Cell Int. 2008 Sep 5;8:12

NCBI and Uniprot Product Information

• NCBI GI #: 115601
• NCBI GeneID: 6275
• UniProt Accession #: P26447
• Molecular Weight: 10-12kDa
• NCBI Official Full Name: Protein S100-A4
• NCBI Official Synonym Full Names: S100 calcium binding protein A4
• NCBI Official Symbol: S100A4
• NCBI Official Synonym Symbols: 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
• NCBI Protein Information: protein S100-A4
• UniProt Protein Name: Protein S100-A4
• UniProt Gene Name: S100A4
• UniProt Synonym Gene Names: CAPL; MTS1
• UniProt Entry Name: S10A4_HUMAN