Mouse anti-Human ATG5 Monoclonal Antibody | anti-ATG5 antibody

ATG5 (Autophagy Marker)

Cat. Num. AAA23947

• Gene Names: ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: ATG5, Antibody; ATG5 (Autophagy Marker); APG5 autophagy 5 like (APG5L); Apoptosis-specific protein (ASP); ATG5; Autophagy related 5; hAPG5; anti-ATG5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: ATG5/2492
• Specificity: The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 275

• Applicable Applications for anti-ATG5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: For coating use Ab at 1-5ug/ml, order Ab without BSA; WB: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: THP1, PANC-1, Raji or HeLa cells. Brain, Ovary, Endometrium, Colon or Duodenum (IHC).
• Immunogen: Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 17p11.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified ATG5 Mouse Monoclonal Antibody (ATG5/2492). Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot)

(Western Blot Analysis of THP1 cell lysate and Brain tissue lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2492).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).)

References

Lanza, F., et al. 1991. J. Biol. Chem. 266: 10638-10645.

NCBI and Uniprot Product Information

• NCBI GI #: 17366828
• NCBI GeneID: 9474
• UniProt Accession #: Q9H1Y0
• Molecular Weight: 50-32kDa
• NCBI Official Full Name: Autophagy protein 5
• NCBI Official Synonym Full Names: autophagy related 5
• NCBI Official Symbol: ATG5
• NCBI Official Synonym Symbols: ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
• NCBI Protein Information: autophagy protein 5
• UniProt Protein Name: Autophagy protein 5
• UniProt Gene Name: ATG5
• UniProt Synonym Gene Names: APG5L; ASP
• UniProt Entry Name: ATG5_HUMAN