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WB (Western Blot) (Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal Antibody.)

Mouse anti-Mouse, Rat HAO1/GOX Monoclonal Antibody | anti-HAO1/GOX antibody

HAO1/GOX (4B4) Mouse mAb

Gene Names
HAO1; GOX; GOX1; HAOX1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Affinity purified
Synonyms
HAO1/GOX; Monoclonal Antibody; HAO1/GOX (4B4) Mouse mAb; Glycolate oxidase; GOX1; HAOX1; Hydroxyacid oxidase 1antibody; MGC142225; anti-HAO1/GOX antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
4B4-3F7-9A6
Specificity
The antibody detects endogenous HAO1 protein.
Purity/Purification
Affinity purified
Form/Format
PBS, pH7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-HAO1/GOX antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:1000-2000
FC/FACS: 1:200
IHC: 1:50-300
Immunogen
Recombinant Protein of HAO1
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Peroxisome
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

WB (Western Blot)

(Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal Antibody.)

WB (Western Blot) (Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal Antibody.)

IF (Immunofluorescence)

(Immunofluorescence analysis of Mouse-spleen tissue. 1,HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-spleen tissue. 1,HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Human-appendix tissue. 1,HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-appendix tissue. 1,HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
Related Product Information for anti-HAO1/GOX antibody
Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
Product Categories/Family for anti-HAO1/GOX antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
hydroxyacid oxidase 1
NCBI Official Synonym Full Names
hydroxyacid oxidase 1
NCBI Official Symbol
HAO1
NCBI Official Synonym Symbols
GOX; GOX1; HAOX1
NCBI Protein Information
hydroxyacid oxidase 1
UniProt Protein Name
Hydroxyacid oxidase 1
UniProt Gene Name
HAO1
UniProt Synonym Gene Names
GOX1; HAOX1; HAOX1; GOX
UniProt Entry Name
HAOX1_HUMAN

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Product Notes

The HAO1/GOX hao1 (Catalog #AAA23994) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HAO1/GOX (4B4) Mouse mAb reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HAO1/GOX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1000-2000 FC/FACS: 1:200 IHC: 1:50-300. Researchers should empirically determine the suitability of the HAO1/GOX hao1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAO1/GOX, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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