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WB (Western Blot)
(C22orf18 monoclonal antibody, Western Blot analysis of C22orf18 expression in K-562.)
Mouse anti-Human CENPM Monoclonal Antibody | anti-CENPM antibody
CENPM (C22orf18, ICEN39, PANE1, Centromere Protein M, Interphase Centromere Complex Protein 39, Proliferation-associated Nuclear Element Protein 1, MGC861, PANE1, BK250D10.2)
Gene Names
CENPM; PANE1; CENP-M; C22orf18; bK250D10.2
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
CENPM; Monoclonal Antibody; CENPM (C22orf18; ICEN39; PANE1; Centromere Protein M; Interphase Centromere Complex Protein 39; Proliferation-associated Nuclear Element Protein 1; MGC861; BK250D10.2); Anti -CENPM (C22orf18; anti-CENPM antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4C12-2C8
Specificity
Recognizes human C22orf18.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL
Applicable Applications for anti-CENPM antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation.
Dilution: Immunofluorescence: 10ug/ml
Dilution: Immunofluorescence: 10ug/ml
Immunogen
Full length recombinant corresponding to aa1-181 from C22orf18 (AAH00705) with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
WB (Western Blot)
(C22orf18 monoclonal antibody, Western Blot analysis of C22orf18 expression in K-562.)
WB (Western Blot)
(C22orf18 monoclonal antibody, Western Blot analysis of C22orf18 expression in K-562.)
Application Data
(Detection limit for recombinant GST tagged C22orf18 is ~0.03ng/ml as a capture antibody.)
Application Data
(Detection limit for recombinant GST tagged C22orf18 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation)
(Immunoprecipitation of CENPM transfected lysate using CENPM monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CENPM rabbit polyclonal antibody.)
IP (Immunoprecipitation)
(Immunoprecipitation of CENPM transfected lysate using CENPM monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CENPM rabbit polyclonal antibody.)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to MGC861 on HeLa cell. [antibody concentration 10ug/ml].)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to MGC861 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot)
(Western Blot analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody.Lane 1: CENPM transfected lysate (19.7kD).Lane 2: Non-transfected lysate.)
WB (Western Blot)
(Western Blot analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody.Lane 1: CENPM transfected lysate (19.7kD).Lane 2: Non-transfected lysate.)
WB (Western Blot)
(Western Blot detection against Immunogen (45.91kD).)
WB (Western Blot)
(Western Blot detection against Immunogen (45.91kD).)
Related Product Information for anti-CENPM antibody
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).
Product Categories/Family for anti-CENPM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,737 Da
NCBI Official Full Name
centromere protein M isoform c
NCBI Official Synonym Full Names
centromere protein M
NCBI Official Symbol
CENPM
NCBI Official Synonym Symbols
PANE1; CENP-M; C22orf18; bK250D10.2
NCBI Protein Information
centromere protein M; interphase centromere complex protein 39; proliferation associated nuclear element 1; proliferation-associated nuclear element protein 1
UniProt Protein Name
Centromere protein M
UniProt Gene Name
CENPM
UniProt Synonym Gene Names
C22orf18; ICEN39; PANE1; CENP-M
UniProt Entry Name
CENPM_HUMAN
Similar Products
Product Notes
The CENPM cenpm (Catalog #AAA24093) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CENPM (C22orf18, ICEN39, PANE1, Centromere Protein M, Interphase Centromere Complex Protein 39, Proliferation-associated Nuclear Element Protein 1, MGC861, PANE1, BK250D10.2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CENPM can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the CENPM cenpm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSVLRPLDKL PGLNTATILL VGTEDALLQQ LADSMLKEDC ASELKVHLAK SLPLPSSVNR PRIDLIVFVV NLHSKYSLQN TEESLRHVDA SFFLGKVCFL ATGAGRESHC SIHRHTVVKL AHTYQSPLLY CDLEVEGFRA TMAQRLVRVL QICAGHVPGV SALNLLSLLR SSEGPSLEDL. It is sometimes possible for the material contained within the vial of "CENPM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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