Mouse anti-Human POLK Monoclonal Antibody | anti-POLK antibody

POLK (DNA Polymerase kappa, DINB1, DINB Protein) (AP)

Cat. Num. AAA24324

• Gene Names: POLK; DINP; POLQ; DINB1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLK, Antibody; POLK (DNA Polymerase kappa, DINB1, DINB Protein) (AP); anti-POLK antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 6F2
• Specificity: Recognizes human POLK.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-POLK antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-473 from human POLK (AAH50718) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKVLYFDMVSLVFKFFNSKMLP
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

WB (Western Blot)

(Western Blot detection against Immunogen (78.03kD).)

WB (Western Blot)

(POLK monoclonal antibody, Western Blot analysis of POLK expression in Hela NE.)

Application Data

(Detection limit for recombinant GST tagged POLK is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence)

(Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry)

(Immunoperoxidase of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)

WB (Western Blot)

(Western Blot analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody. Lane 1: POLK transfected lysate (54.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot)

(Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Product Categories/Family for anti-POLK antibody

Antibodies; Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 30048186
• NCBI GeneID: 51426
• Molecular Weight: 88,342 Da
• NCBI Official Full Name: Homo sapiens polymerase (DNA directed) kappa, mRNA
• NCBI Official Synonym Full Names: DNA polymerase kappa
• NCBI Official Symbol: POLK
• NCBI Official Synonym Symbols: DINP; POLQ; DINB1
• NCBI Protein Information: DNA polymerase kappa