Mouse anti-Human DUSP6 Monoclonal Antibody | anti-DUSP6 antibody

DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) (FITC)

Cat. Num. AAA25082

• Gene Names: DUSP6; HH19; MKP3; PYST1
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DUSP6, Antibody; DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) (FITC); anti-DUSP6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3G2
• Specificity: Recognizes human DUSP6.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-DUSP6 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-381 from human DUSP6 (AAH03143) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MIDTLRPVPFASEMAISKTVAWLNEQLELGNERLLLMDCRPQELYESSHIESAINVAIPGIMLRRLQKGNLPVRALFTRGEDRDRFTRRCGTDTVVLYDESSSDWNENTGGESLLGLLLKKLKDEGCRAFYLEGGFSKFQAEFSLHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDIESDLDRDPNSATDSDGSPLSNSQPSFPVEILPFLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFIDEARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQQLYFTTPSNQNVYQVDSLQST
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

WB (Western Blot)

(Western blot analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data

(Detection limit for recombinant GST tagged DUSP6 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence)

(Immunofluorescence of monoclonal antibody to DUSP6 on HeLa cell. [antibody concentration 10ug/ml.)

IHC (Immunohistochemistry)

(Immunoperoxidase of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml].)

WB (Western Blot)

(Western Blot analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody. Lane 1: DUSP6 transfected lysate (42kD). Lane 2: Non-transfected lysate.)

WB (Western Blot)

(Western Blot detection against Immunogen (67.65kD).)

Product Categories/Family for anti-DUSP6 antibody

Antibodies; Enzymes; Phosphatase

References

1. Dual specificity phosphatase 6 as a predictor of invasiveness in papillary thyroid cancer. Lee JU, Huang S, Lee MH, Lee SE, Ryu MJ, Kim SJ, Kim YK, Kim SY, Joung KH, Kim JM, Shong M, Jo YS.Eur J Endocrinol. 2012 Jul;167(1):93-101. Epub 2012 Apr 25. 2. Down-Regulation of DUSP6 Expression in Lung Cancer: Its Mechanism and Potential Role in Carcinogenesis. Okudela K, Yazawa T, Woo T, Sakaeda M, Ishii J, Mitsui H, Shimoyamada H, Sato H, Tajiri M, Ogawa N, Masuda M, Takahashi T, Sugimura H, Kitamura H.Am J Pathol. 2009 Aug;175(2):867-81. Epub 2009 Jul 16.

NCBI and Uniprot Product Information

• NCBI GI #: 13111942
• NCBI GeneID: 1848
• Molecular Weight: 26,468 Da
• NCBI Official Full Name: Homo sapiens dual specificity phosphatase 6, mRNA
• NCBI Official Synonym Full Names: dual specificity phosphatase 6
• NCBI Official Symbol: DUSP6
• NCBI Official Synonym Symbols: HH19; MKP3; PYST1
• NCBI Protein Information: dual specificity protein phosphatase 6; MAP kinase phosphatase 3; dual specificity protein phosphatase PYST1; mitogen-activated protein kinase phosphatase 3; serine/threonine specific protein phosphatase