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IP (Immunoprecipitation)
(Immunoprecipitating GPI in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA26916 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26916 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
Rabbit anti-Human Glucose-6-phosphate isomerase Polyclonal Antibody | anti-GPI antibody
Rabbit anti-human Glucose-6-phosphate isomerase polyclonal Antibody
Gene Names
GPI; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Glucose-6-phosphate isomerase, Antibody; Rabbit anti-human Glucose-6-phosphate isomerase polyclonal Antibody; Autocrine motility factor; Phosphoglucose isomerase; Phosphohexose isomerase; Sperm antigen 36; anti-GPI antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
558
Applicable Applications for anti-GPI antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Recommended dilution: IHC: 1:20-1:200
Immunogen
Recombinant human Glucose-6-phosphate isomerase
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30392 / sc-33777
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IP (Immunoprecipitation)
(Immunoprecipitating GPI in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA26916 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26916 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
IP (Immunoprecipitation)
(Immunoprecipitating GPI in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA26916 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26916 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
IF (Immunofluorescence)
(Immunofluorescent analysis of PC3 cells using AAA26916 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of PC3 cells using AAA26916 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human endometrial cancer using AAA26916 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human endometrial cancer using AAA26916 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA26916 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA26916 at dilution of 1:100)
WB (Western Blot)
(Western BlotPositive WB detected in: PC3 whole cell lysate,Hela whole cell lysate,Rat brain tissue,Mouse heart tissue,Mouse liver tissue,Mouse kidney tissue,Mouse brain tissueAll lanes: GPI antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64 KDaObserved band size: 64 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: PC3 whole cell lysate,Hela whole cell lysate,Rat brain tissue,Mouse heart tissue,Mouse liver tissue,Mouse kidney tissue,Mouse brain tissueAll lanes: GPI antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64 KDaObserved band size: 64 KDa)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embeded human kidney using AAA26916 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embeded human kidney using AAA26916 at dilution of 1:100)
Related Product Information for anti-GPI antibody
Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. This protein is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. This protein is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Product Categories/Family for anti-GPI antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,325 Da
NCBI Official Full Name
glucose-6-phosphate isomerase isoform 2
NCBI Official Synonym Full Names
glucose-6-phosphate isomerase
NCBI Official Symbol
GPI
NCBI Official Synonym Symbols
AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
NCBI Protein Information
glucose-6-phosphate isomerase
UniProt Protein Name
Glucose-6-phosphate isomerase
UniProt Gene Name
GPI
UniProt Synonym Gene Names
GPI; AMF; NLK; PGI; PHI; SA-36
UniProt Entry Name
G6PI_HUMAN
Similar Products
Product Notes
The GPI gpi (Catalog #AAA26916) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Glucose-6-phosphate isomerase polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glucose-6-phosphate isomerase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Recommended dilution: IHC: 1:20-1:200. Researchers should empirically determine the suitability of the GPI gpi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucose-6-phosphate isomerase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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