Mouse Hyaluronan and proteoglycan link protein 1 (HAPLN1) ELISA Kit | HAPLN1 elisa kit

Mouse Hyaluronan and proteoglycan link protein 1 (HAPLN1) ELISA Kit

Cat. Num. AAA27105

• Gene Names: HAPLN1; CRTL1
• Reactivity: Mouse
• Synonyms: Hyaluronan and proteoglycan link protein 1 (HAPLN1); N/A; Mouse Hyaluronan and proteoglycan link protein 1 (HAPLN1) ELISA Kit; HAPLN1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of HAPLN1. No significant cross-reactivity or interference between HAPLN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HAPLN1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for HAPLN1 elisa kit

Principle of the Assay: HAPLN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HAPLN1 antibody and an HAPLN1-HRP conjugate. The assay sample and buffer are incubated together with HAPLN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HAPLN1 concentration since HAPLN1 from samples and HAPLN1-HRP conjugate compete for the anti-HAPLN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HAPLN1 from the sample, fewer sites are left to bind HAPLN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HAPLN1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This HAPLN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HAPLN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for HAPLN1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4503053
• NCBI GeneID: 1404
• NCBI Accession #: NP_001875.1
• NCBI GenBank Nucleotide #: NM_001884.3
• Molecular Weight: 40,166 Da
• NCBI Official Full Name: hyaluronan and proteoglycan link protein 1
• NCBI Official Synonym Full Names: hyaluronan and proteoglycan link protein 1
• NCBI Official Symbol: HAPLN1
• NCBI Official Synonym Symbols: CRTL1
• NCBI Protein Information: hyaluronan and proteoglycan link protein 1; Cartilage link protein; cartilage-link protein; cartilage linking protein 1; cartilage-linking protein 1
• UniProt Protein Name: Hyaluronan and proteoglycan link protein 1
• UniProt Gene Name: HAPLN1
• UniProt Synonym Gene Names: CRTL1; Cartilage-link protein
• UniProt Entry Name: HPLN1_HUMAN